Antibacterial Efficacy of Turmeric (Curcuma domestica) Rhizome Infusion Against Aeromonas hydrophila and Its Toxicity
DOI:
https://doi.org/10.21580/ah.v6i2.16934Keywords:
Aeromonas hydrophila, Brine Shrimp, Curcuma domestica, Infusion, TurmericAbstract
Aeromonas hydrophila bacteria can cause Motile Aeromonas Septicemia (MAS), a disease that impacts freshwater fish. The turmeric rhizome contains numerous bioactive compounds that act as antibacterials and might be utilized to inhibit A. hydrophila. This research aimed to identify the phytochemicals in turmeric (Curcuma domestica) infusion, evaluate its antibacterial activity against A. hydrophila, and define the toxicity effect of turmeric rhizome infusion. The agar dilution method was used to measure antibacterial activity, whereas the Brine Shrimp Lethality Test (BSLT) was used to assess toxicity. The research confirmed the presence of phytochemicals such as tannins, alkaloids, flavonoids, phenols, and saponins in a turmeric rhizome infusion. According to the findings, the infusion from turmeric rhizome effectively inhibited the growth of A. hydrophila at concentrations between 750-1000 ppm. In toxicity tests, the LC50 value of the turmeric rhizome infusion against shrimp larvae was 381.18 ppm. Hence, the turmeric rhizome infusion has the potential to be further developed for the prevention and treatment of fish infected with A. hydrophila in freshwater fish farming.Downloads
References
Adamczak, A., Ożarowski, M., & Karpiński, T. M. (2020). Curcumin, a Natural Antimicrobial Agent with Strain-Specific Activity. Pharmaceuticals, 13(7), 153. https://doi.org/10.3390/ph13070153
Antara. (2018, February 23). "14 ribu ikan gurami mati akibat bakteri aeromonas." Antara News. Retrieved from https://jateng.antaranews.com/berita/189846/14-ribu-ikan-guramimati-akibat-bakteri-aeromonas.
Banti, C. N., & Hadjikakou, S. K. (2021). Evaluation of Toxicity with Brine Shrimp Assay. Bio-protocol, 11(2), e3895. https://doi.org/10.21769/BioProtoc.3895
Bariyyah, S.K., Prajitno, A., & Yuniarti, A. (2019). Phytochemical screening and antimicrobial activity of Roselle (Hibiscus sabdariffa L) flower extract against Aeromonas hydrophila. Journal of Experimental and Life Sciences, 9(2), 1-6. https://doi.org/10.21776/ub.jels.2019.009.02.01
Beaz-Hidalgo, R., & Figueras, M. J. (2013). Aeromonas spp. whole genomes and virulence factors implicated in fish disease. Journal of Fish Diseases, 36(4), 371-388. https://doi.org/10.1111/jfd.12025
Cobra, L., Shella, H., Helda, W. A., & Amalia, E. (2019). Skirining Fitokimia Ekstrak Sokhletasi Rimpang Kunyit (Curcuma longa) dengan Pelarut Etanol 96%. Jurnal Ilmilah Putra Bangsa, 1, 12-17.
Dewi, S. (2011). Jurus Tepat Budidaya Ikan Patin. Yogyakarta: Pustaka Baru Press.
Ecevit, K., Barros, A. A., Silva, J. M., & Reis, R. L. (2022). Preventing Microbial Infections with Natural Phenolic Compounds. Future Pharmacology, 2(4), 460-498. https://doi.org/10.3390/futurepharmacol2040030
Grover, M., Behl, T., Sehgal, A., Singh, S., Sharma, N., Virmani, T., Rachamalla, M., Farasani, A., Chigurupati, S., Alsubayiel, A. M., Felemban, S. G., Sanduja, M., & Bungau, S. (2021). In vitro Phytochemical Screening, Cytotoxicity Studies of Curcuma longa Extracts with Isolation and Characterisation of Their Isolated Compounds. Molecules, 26(24), 7509. https://doi.org/10.3390/molecules26247509
Hamidi, M. R., Jovanova, B., & Panovska, T. K. (2014). Toxicological evaluation of the plant products using Brine Shrimp (Artemia salina L.) model. Macedonian Pharmaceutical Bulletin, 60(1), 11-17. https://doi.org/10.33320/maced.pharm.bull.2014.60.01.002
Hardi, I., Wijaya, K., Suwinarti, W., Agustina, A., & Nugroho, R. A. (2016). Antibacterial activity of Boesenbergia pandurata, Zingiber zerumbet and Solanum ferox extracts against Aeromonas hydrophila and Pseudomonas sp. Journal Nusantara Bioscience, 8(1), 18-21. https://doi.org/10.13057/nusbiosci/n080105
Hewlings, S. J., & Kalman, D. S. (2017). Curcumin: A review of its effects on human health. Foods, 6(10), 92. https://doi.org/10.3390/foods6100092
Jamil, S., Khan, R. A., Afroz, S., & Ahmed, S. (2016). Phytochemistry, brine shrimp lethality and mice acute oral toxicity studies on seed extracts of Vernonia anthelmintica. Pakistan Journal of Pharmaceutical Sciences, 29(6), 2053-2057. PMID: 28375123
Kaczmarek, B. (2020). Tannic acid with antiviral and antibacterial activity as a promising component of biomaterials-a minireview. Materials, 13(14), 3224. https://doi.org/10.3390/ma13143224
Kari, Z. A., Wee, W., Sukri, S. A. M., Harun, H. C., Reduan, M. F. H., Khoo, M. I., Doan, H. V., Goh, K. W., & Lee, S. W. (2022). Role of phytobiotics in relieving the impacts of Aeromonas hydrophila infection on aquatic animals: A mini-review. Frontiers in Veterinary Science, 9, 1023784. https://doi.org/10.3389/fvets.2022.1023784
Karmila, U., Karina, S., & Yulfizar, C. (2017). Ekstrak kunyit (Curcuma domestica) sebagai anti bakteri Aeromonas hydrophyla pada ikan Patin (Pangasius sp.). Jurnal Ilmiah Mahasiswa Kelautan dan Perikanan Unsyiah, 2(1), 150-157.
Kenconojati, H., & Rukmana, N. R. (2019). Daya hambat ekstrak daun kelor (Moringa oleifera) terhadap Aeromonas hydrophila: Studi awal untuk pengobatan aeromoniasis. Journal of Aquaculture Science, 4(1), 12-20. https://doi.org/10.31093/joas.v4i1.64
Khan, M. I., Ahhmed, A., Shin, J. H., Baek, J. S., Kim, M. Y., & Kim, J. D. (2018). Green Tea Seed Isolated Saponins Exerts Antibacterial Effects against Various Strains of Gram Positive and Gram Negative Bacteria, a Comprehensive Study In Vitro and In Vivo. Evidence-based Complementary and Alternative Medicine, 2018, 3486106. https://doi.org/10.1155/2018/3486106
Khasanah, N., Wakidatul, B., Bhakti, K., Karyadi, & Sundaryono, A. (2020). Uji Fitokimia dan Toksisitas Ekstrak Umbi Hydnophytum sp. Terhadap Artemia salina Leach. Journal of Science Education, 4(1), 47-53. https://doi.org/10.33369/pendipa.4.1.47-53
Manyi-Loh, C., Mamphweli, S., Meyer, E., & Okoh, A. (2018). Antibiotic Use in Agriculture and Its Consequential Resistance in Environmental Sources: Potential Public Health Implications. Molecules, 23(4), 795. https://doi.org/10.3390/molecules23040795
Nur, S., Mubarak, F., Jannah, C., Winarni, D. A., Rahman, D. A., Hamdayani, L. A., ... & Muharni. (2019). Total phenolic and flavonoid compounds, antioxidant and toxicity profile of extract and fraction of Paku atai tuber (Angiopteris ferox Copel). Food Research, 3(6), 734-740. https://doi.org/10.26656/fr.2017.3(6).135
Pękala-Safińska, A., Tkachenko, H., Kurhaluk, N., Buyun, L., Osadowski, Z., Honcharenko, V., & Prokopiv, A. (2021). Studies on the Inhibitory Properties of Leaf Ethanolic Extracts Obtained from Ficus (Moraceae) Species Against Aeromonas Spp. Strains. Journal of Veterinary Research, 65(1), 59-66. https://doi.org/10.2478/jvetres-2021-0007
Polianciuc, S. I., Gurzău, A. E., Kiss, B., Ştefan, M. G., & Loghin, F. (2020). Antibiotics in the environment: causes and consequences. Medicine and Pharmacy Reports, 93(3), 231-240. https://doi.org/10.15386/mpr-1742
Rasmussen-Ivey, C. R., Figueras, M. J., McGarey, D., & Liles, M. R. (2016). Virulence factors of Aeromonas hydrophila: in the wake of reclassification. Frontiers in Microbiology, 7, 1337. https://doi.org/10.3389/fmicb.2016.01337
Riauwaty, M., Siregar, Y. I., & Mulyani, I. (2021). Effectiveness of turmeric-enriched pellets to improve the immunity of Clarias batrachus toward motile Aeromonas septicemia disease. F1000Research, 10, 169. https://doi.org/10.12688/f1000research.28260.2
Rosidah, Ibnu Dwi Buwono, Walim Lili, Ibnu Bangkit Suryadi, & Ade Reza Triandika. (2018). Ketahanan ikan lele sangkuriang, Clarias gariepinus Burchell 1822 terhadap Aeromonas hydrophyla pasca pemberian ekstrak daun kelor (Moringa oleifera L.) melalui pakan. Jurnal Iktiologi Indonesia, 19(1), 97-113. https://doi.org/10.32491/jii.v19i1.435
Sari, E. T. P., Gunaedi, T., & Indrayani, E. (2017). Pengendalian infeksi bakteri Aeromonas hydrophyla pada ikan Nila (Oreochromis niloticus) dengan ekstrak rimpang lengkuas merah (Alpinia purpurata). Jurnal Biologi Papua, 9(2), 37-42. https://doi.org/10.31957/jbp.110
Semwal, A., Kumar, A., & Kumar, N. (2020). A review on pathogenicity of Aeromonas hydrophila and their mitigation through medicinal herbs in aquaculture. SSRN. https://doi.org/10.2139/ssrn.4298008
Setyowati, W. A. E., Ariani, S. R. D., Ashadi, Mulyani, B., & Rahmawati, C. P. (2014). Skrining Fitokimia dan Identifikasi Komponen Utama Ekstrak Metanol Kulit Durian (Durio zibethinus Murr.) Varietas Petruk. In Seminar Nasional Kimia dan Pendidikan Kimia UNS.
Sharifi, S., Fathi, N., Memar, M. Y., Khatibi, S. M. H., Khalilov, R., Negahdari, R., Vahed, S. Z., & Dizaj, S. M. (2020). Antimicrobial activity of curcumin nanoformulations: New trends and future perspectives. Phytotherapy Research. https://doi.org/10.1002/ptr.6658.
Sharififar, F., Assadipour, A., Moshafi, M. H., Alishahi, F., & Mahmoudvand, H. (2017). Bioassay screening of the essential oil and various extracts of Nigella sativa L. Seeds using brine shrimp toxicity assay. Herbal Medicine Journal, 2(1), 26-31.
Sharifi-Rad, J., Rayess, Y. E., Rizk, A. A., Sadaka, C., Zgheib, R., Zam, W., Sestito, S., Rapposelli, S., Neffe-Skocińska, K., Zielińska, D., Salehi, B., Setzer, W. N., Dosoky, N. S., Taheri, Y., El Beyrouthy, M., Martorell, M., Ostrander, E. A., Suleria, H. A. R., Cho, W. C., Maroyi, A., … Martins, N. (2020). Turmeric and Its Major Compound Curcumin on Health: Bioactive Effects and Safety Profiles for Food, Pharmaceutical, Biotechnological and Medicinal Applications. Frontiers in Pharmacology, 11, 1021. https://doi.org/10.3389/fphar.2020.01021
Stratev, D., & Odeyemi, O. A. (2017). An overview of motile Aeromonas septicaemia management. Aquaculture International, 25, 1095-1105. https://doi.org/10.1007/s10499-016-0100-3
Tanhay Mangoudehi, H., Zamani, H., Shahangian, S. S., & Mirzanejad, L. (2020). Effect of curcumin on the expression of ahyI/R quorum sensing genes and some associated phenotypes in pathogenic Aeromonas hydrophila fish isolates. World Journal of Microbiology & Biotechnology, 36(5), 70. https://doi.org/10.1007/s11274-020-02846-x
Tyagi, P., Singh, M., Kumari, H., Kumari, A., & Mukhopadhyay, K. (2015). Bactericidal activity of curcumin I is associated with damaging of bacterial membrane. PLoS ONE, 10, e0121313. https://doi.org/10.1371/journal.pone.0121313
Wardani, R. K., Wahju, T., & Budi, S. R. (2012). Uji Pengaruh Ekstrak Daun Sirih Merah (Piper rocatum) terhadap bakteri Aeromonas hydrophila secara In Vitro. Jurnal Ilmiah dan Kelautan, 4, 59-64. https://doi.org/10.20473/jipk.v4i1.11584
Woo, S.-J., Kim, M.-S., Jeong, M.-G., Do, M.-Y., Hwang, S.-D., & Kim, W.-J. (2022). Establishment of Epidemiological Cut-Off Values and the Distribution of Resistance Genes in Aeromonas hydrophila and Aeromonas veronii Isolated from Aquatic Animals. Antibiotics, 11(3), 343. https://doi.org/10.3390/antibiotics11030343
Xie, Y., Yang, W., Tang, F., Chen, X., & Ren, L. (2015). Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Current medicinal chemistry, 22(1), 132-149. https://doi.org/10.2174/0929867321666140916113443
Yan, Y., Li, X., Zhang, C., Lv, L., Gao, B., & Li, M. (2021). Research Progress on Antibacterial Activities and Mechanisms of Natural Alkaloids: A Review. Antibiotics, 10(3), 318. https://doi.org/10.3390/antibiotics10030318
Zheng, D., Huang, C., Huang, H., Zhao, Y., Khan, M. R. U., Zhao, H., & Huang, L. (2020). Antibacterial Mechanism of Curcumin: A Review. Chemistry & Biodiversity. https://doi.org/10.1002/cbdv.202000171
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