Karakteristik dan Potensi Enzimatis Bakteri Asal Tanah Sampah Dapur dan Kotoran Ternak sebagai Kandidat Agen Biodegradasi Sampah Organik

Taruna Dwi Satwika*  -  Universitas Jenderal Soedirman, Indonesia
Dwiana Muflihah Yulianti  -  Fakultas Biologi Universitas Jenderal Soedirman Purwokerto, Indonesia
Arif Rahman Hikam  -  Fakultas Biologi Universitas Jenderal Soedirman Purwokerto, Indonesia

(*) Corresponding Author

Composting is an alternative for recycling organic waste. Microorganisms that can degrade the components of organic waste are an essential part of composting. Isolation and selection of bacteria with the ability to degrade the elements of organic waste are the first steps to obtain an organic waste degrading agent. This study aimed to determine the characteristics and the enzymatic potential (cellulolytic, amylolytic, and proteolytic) of bacteria isolated from soil, cow dung and kitchen waste as candidates for organic waste degradation agents. The research begins with sampling and isolation of bacteria. The isolates obtained were tested qualitatively for their amylolytic, cellulolytic and proteolytic activities. Furthermore, bacterial isolates were characterized morphologically and biochemically. A total of 30 bacterial isolates were isolated from soil samples, cow dung and kitchen waste. The screening result showed that 6 isolates had amylolytic activity, 7 isolates had cellulolytic activity and 3 isolates had proteolytic activity. These bacterial isolates showed various morphological and biochemical characteristics. In general, soil samples, kitchen waste and cow dung contain potential bacteria as organic waste degrading agents

Keywords : Amylolytic bacteria; Cellulolytic bacteria; Organic waste; Proteolytic bacteria

  1. Akhtar, N., Sharma, A., Deka, D., Jawed, M., Goyal, D., & Goyal, A. (2013). Characterization of cellulase producing Bacillus sp. for effective degradation of leaf litter biomass. Environmental Progress and Sustainable Energy. https://doi.org/10.1002/ep.11726
  2. Awan, K., Jabeen, F., Manzoor, M., & Qazi, J. I. (2018). Potential of thermophilic amylolytic bacteria for growth in unconventional media: Potato peels. Journal of Food Process Engineering, 41(1), 1–7. https://doi.org/10.1111/jfpe.12635
  3. Chahar, P. J., & Prakash, A. (2017). Isolation and Molecular Characterization of Cellulolytic Microbes From Cow Dung. 6(1), 1–4.
  4. Chiarelotto, M., Bottin, W. C., Spicker, C. E., Duarte, S. S., Chiarelotto, M., & Bortoli, M. M. (2018). Composting of household organic waste: effect on control parameters and final compound quality. Revista Agro@Mbiente On-Line, 12(4), 272. https://doi.org/10.18227/1982-8470ragro.v12i4.5126
  5. Echeverria, M. C., Cardelli, R., Bedini, S., Agnolucci, M., Cristani, C., Saviozzi, A., & Nuti, M. (2011). Composting Wet Olive Husks with a Starter Based on Oil-Depleted Husks Enhances Compost Humification. Compost Science and Utilization, 19(3), 182–188. https://doi.org/10.1080/1065657X.2011.10736998
  6. Hasan, M. M., Marzan, L. W., Hosna, A., Hakim, A., & Azad, A. K. (2017). Optimization of some fermentation conditions for the production of extracellular amylases by using Chryseobacterium and Bacillus isolates from organic kitchen wastes. Journal of Genetic Engineering and Biotechnology, 15(1), 59–68. https://doi.org/10.1016/j.jgeb.2017.02.009
  7. Islam, F., & Roy, N. (2018). Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses. BMC Research Notes. https://doi.org/10.1186/s13104-018-3558-4
  8. Jabir, D. M., & Jabir, M. M. (2016). A study of biodegradation of paper wastes by using bacteria isolated from the soil. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 18(3), 777–780. https://doi.org/10.13140/RG.2.2.33601.97124
  9. Jini, R., Swapna, H. C., Rai, A. K., Vrinda, R., Halami, P. M., Sachindra, N. M., & Bhaskar, N. (2011). Isolation and characterization of potential lactic acid bacteria (LAB) from freshwater fish processing wastes for application in fermentative utilisation of fish processing waste. Brazilian Journal of Microbiology. https://doi.org/10.1590/S1517-83822011000400039
  10. Krishna, M. P., & Mohan, M. (2017). Litter decomposition in forest ecosystems: a review. Energy, Ecology and Environment, 2(4), 236–249. https://doi.org/10.1007/s40974-017-0064-9
  11. Lal, A., & Cheeptham, N. (2012). Starch agar protocol. American Society for Microbiology.
  12. Liang, Y. L., Zhang, Z., Wu, M., Wu, Y., & Feng, J. X. (2014). Isolation, screening, and identification of cellulolytic bacteria from natural reserves in the subtropical region of China and optimization of cellulase production by Paenibacillus terrae ME27-1. BioMed Research International. https://doi.org/10.1155/2014/512497
  13. Madigan, M., Martinko, J. M., Bender, K. S., Buckley, D. H., & Stahl, D. A. (2015). Brock: Biology of the Microorganisms. In Journal of Chemical Information and Modeling.
  14. Maki, M. L., Idrees, A., Leung, K. T., & Qin, W. (2012). Newly isolated and characterized bacteria with great application potential for decomposition of lignocellulosic biomass. Journal of Molecular Microbiology and Biotechnology, 22(3), 156–166. https://doi.org/10.1159/000341107
  15. Mazzucotelli, C. A., Ponce, A. G., Kotlar, C. E., & Moreira, M. del R. (2013). Isolation and characterization of bacterial strains with a hydrolytic profile with potential use in bioconversion of agroindustial by-products and waste. Food Science and Technology, 33(2), 295–303. https://doi.org/10.1590/S0101-20612013005000038
  16. Mishra, S., & Behera, N. (2008). Amylase activity of a starch degrading bacteria isolated from soil receiving kitchen wastes. African Journal of Biotechnology, 7(18), 3326–3331. https://doi.org/10.5897/AJB08.582
  17. Rashid, G. M. M., Durán-Peña, M. J., Rahmanpour, R., Sapsford, D., & Bugg, T. D. H. (2017). Delignification and enhanced gas release from soil containing lignocellulose by treatment with bacterial lignin degraders. Journal of Applied Microbiology. https://doi.org/10.1111/jam.13470
  18. Saha, M. L., Islam, K. N., Akter, T., Rahman, I. A., Islam, T., & Khan, T. (2019). Isolation and identification of amylolytic bacteria from garbage and garden soil. Bangladesh Journal of Botany. https://doi.org/10.3329/BJB.V48I3.47915
  19. Sarkar, P., Meghvanshi, M., & Singh, R. (2011). Microbial Consortium: A New Approach in Effective Degradation of Organic Kitchen Wastes. International Journal of Environmental Science and Development. https://doi.org/10.7763/ijesd.2011.v2.118
  20. Silaban, S., Marika, D. B., & Simorangkir, M. (2020). Isolation and characterization of amylase-producing amylolytic bacteria from rice soil samples. Journal of Physics: Conference Series, 1485(1). https://doi.org/10.1088/1742-6596/1485/1/012006
  21. Soundra Josephine, F., S, R. V, Devi, N., Babu Ganapa, S., & G, S. K. (2012). Isolation, production and characterization of protease from Bacillus Sp isolated from soil sample. Journal of Microbiology and Biotechnology Research Scholars Research Library J. Microbiol. Biotech. Res.
  22. Utomo, M. A. P. and S. M. (2014). Bakteri Tanah Pendegradasi Bahan Organik. 3(2), 80–83.
  23. Woo, H. L., Hazen, T. C., Simmons, B. A., & DeAngelis, K. M. (2014). Enzyme activities of aerobic lignocellulolytic bacteria isolated from wet tropical forest soils. Systematic and Applied Microbiology, 37(1), 60–67. https://doi.org/10.1016/j.syapm.2013.10.001

Open Access Copyright (c) 2021 Al-Hayat: Journal of Biology and Applied Biology
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.