Potential of Essential Oil Eucalyptus botryoides Leaves as an Antibacterial in Hand Sanitizer

Andi Nur Fitriani Abubakar*  -  Department of Chemistry, Faculty of Sciences, Universitas Muhammadiyah Bulukumba, Indonesia
Syahdam Karneng  -  Department of Chemistry, Faculty of Sciences, Universitas Muhammadiyah Bulukumba, Indonesia
Firnanelty Firnanelty  -  Department of Chemistry, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar, Indonesia
Rezki Nur Damayanti  -  Department of Chemistry, Faculty of Sciences, Universitas Muhammadiyah Bulukumba, Indonesia
St. Aisyah  -  Department of Chemistry, Faculty of Sciences, Universitas Muhammadiyah Bulukumba, Indonesia
Ahmad Bikharuddin  -  Graduate School of Medicine, Dentistry and Pharmaceutical Scinces, Okayama University, Japan

(*) Corresponding Author
DOI: 10.21580/wjc.v5i2.13292

Hand sanitizer is an antiseptic product that is widely used to prevent the spread of bacteria and viruses. Hand sanitizer preparations circulating in the market still use alcohol as an antibacterial agent. One of the natural antibacterial Eucalyptus is an antibacterial agent, and essential oil extracted from Eucalyptus can be named as natural ingredient. The purpose of this study was to determine the potential of essential oil E. botryoides leaves as an antibacterial ingredient to make of hand sanitizer. Eucalyptus essential oil is extracted from its leave using steam distillation method and formulated in the making of hand sanitizer gel with various concentrations of 2%, 4%, and 6%. The formulation of hand sanitizer gel was tested for antibacterial, organoleptic, homogeneity, pH, and dispersibility. The results showed that the more concentration of essential oil from Eucalyptus botryoides leaves added to the hand sanitizer gel, the more its antibacterial activity increased. The results of organoleptic observations, homogeneity, pH, and dispersibility of hand sanitizer gel preparations have met the requirements of SNI No.06-2588-1992. Thus, essential oil from E. botryoides leaves has potential as an antibacterial in hand sanitizer gel products.

Keywords: Essential oil; Eucalyptus botryoides; antibacterial; hand sanitizer

  1. Ammer, M.R., Shah, Z., Muhammad, K., Muhammad, B., Shazia, E., Danfeng, H., Shengquan, C., 2016, Optimization of antibacterial activity of Eucalyptus tereticornis leaf extracts against Escherichia coli through response surface methodology, Journal of Radiation Research and Applied Sciences, 376-385.
  2. Astiani, D.P., Jayuska, A., Arreneuz, S., 2014, Uji Aktivitas Antibakteri Minyak Atsiri Eucalyptus pellita Terhadap Bakteri Escherichia coli Dan Staphylococcus aureus, JKK, 3(3), 49-53.
  3. Bachir, R.G., Benali M, 2012, Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus, Asian Pacific Journal of Tropical Biomedicine, 2(9), 739-742.
  4. Bhuyan, D.J., Q.V, Vuong., A.C, Chaimers., L.A, Van, A., M.C, Bowyer., C.J, Scarlett., 2017, Phytochemical, antibacterial and antifungal properties of an aqueous extract of Eucalyptus microcorys leaves, South African Journal of Botany, 180-185.
  5. Davis, W.W., T.R, Stout, 1971, Disc Plate Method of Microbiological Antibiotic Assay, American Society for Microbiology, 2(4), 659-665.
  6. Elaissi, A., Rouis, Z., Salem, N.A.B., Mabrouk, S., Salem, Y.B., Salah, K.B.H., Aounis, M., Farhat, F., Chemli, R., Skhiri, F.H., Khouja, M.L., 2012, Chemical composition of 8 eucalyptus species' essential oils and the evaluation of their antibacterial, antifungal and antiviral activities, BMC Complementary and Alternative Medicine, 12(81), 1-15.
  7. Handayani, S.N., Purwanti, A., Windasari, Ardian, M.N., 2020, Uji Fitokimia dan Aktivitas Ekstrak Etanol Daun Kencana Ungu (Ruellita tuberosa L.), Walisongo Journal of Chemistry, 3(2), 66-70. https://doi.org/10.21580/wjc.v3i2.6119.
  8. Khammassi, M., Flavio, P., Ismail, A., Sana, K., Lamia, H., Filomena, N., Florinda, F., Vincenzo, D.F., 2022, Chemical Composition and Phytotoxic, Antibacterial and Antibiofilm Activity of the Essential Oils of Eucalyptus occidentalis, E. striaticalyx and E. stricklandii, Molecules, 27, 5820, https://doi.org/10.3390/molecules27185820.
  9. Matondang, A.F., 2019, Uji Aktivitas Antibakteri Gel Hand Sanitizer Minyak Atsiri Daun Eukaliptus (Eucalyptus camaldulensis Dehnh.) Terhadap Staphylococcus aureus dan Staphylococcus epidermidis, Skripsi, Universitas Sumatera Utara Medan.
  10. Miguel, M.G., Custódia, G., Maria, D.A., Soraia, L., Maria, L.F., Cristina, M., Isabel, C.G., Javier, V., Ana, C.F., 2018, Antibacterial, Antioxidant, and Antiproliferative Activities of Corymbia citriodora and the Essential Oils of Eight Eucalyptus Species, Medicines, 5, 61, doi:10.3390/medicines5030061.
  11. Octavia, N., 2016, Formulasi Sediaan Gel Hand Sanitizer Minyak Atsiri Pala (Myristica Fragranshoutt.) : Uji Stabilitas Fisik Dan Uji Aktivitas Antibakteri Terhadap Bakteri Staphylococcus aureus, Skripsi, Universitas Muhammadiyah Surakarta.
  12. Putri, M.A., Saputra, M.E., Amanah, I.N., Musiam, S., Fabiani, V.A., 2020, Hand Sanitizer Ekstrak Daun Pucuk Idat (Cratoxylum glaucum) sebagai Antibakteri Staphycocus aureus dan Escherichia coli, ALCHEMY Jurnal Penelitian Kimia, 16(2), 227-231.
  13. Rohmani, S., Kuncoro, M.A.A., 2019, Uji Stabilitas dan Aktivitas Gel Handsanitizer Ekstrak Daun Kemangi, Journal of Pharmaceutical Science and Clinical Research, 01, 16-28.
  14. Sebei, K., Sakouhi, F., Herchi, W., Khouja, M.L., Boukhchina, S., 2015, Chemical composition and antibacterial activities of seven Eucalyptus species essential oils leaves, Biological Research, 48(7), 1-5.
  15. Standar Nasional Indonesia, 1992, Detergen Sintetik Cair Pembersih Tangan, Badan Standarisasi Nasional, No 06-2588.
  16. Tong, S.Y.C., Joshua, S.D., Emily, E., Thomas, L.H., Vance, G.F., Jr., 2015, Staphylococcus aureus Infections: Epidemiology, Pathophysiology, Clinical Manifestations, and Management, Journal Clinical Microbiology Reviews, 28(3) doi:10.1128/CMR.00134-14.
  17. Widyasanti, A., Shalsabilla, R.T., 2020, Karakterisasi Sediaan Antiseptik Gel Handmade Dengan Penambahan Bahan Aktif Minyak Atsiri Eucalytus Dan Grapefruit, Agroindustrial Technology Journal, 04(02), 136-144. http://dx.doi.org/10.21111/atj.v4i2.4965

Open Access Copyright (c) 2022 Walisongo Journal of Chemistry
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

WJC: Walisongo Journal of Chemistry
Published by the Department of Chemistry
Faculty of Science and Technology
Universitas Islam Negeri Walisongo Semarang
Jl Prof. Dr. Hamka Kampus III Ngaliyan Semarang 50185
Website: https://journal.walisongo.ac.id/index.php/wjc
Email: [email protected]  [email protected]

ISSN: 2549-385X (Print)
ISSN: 2621-5985 (Online)




 

apps