Comparative Studies of the Phytochemical Analysis, Mineral Content, Antibacterial Activity and Antioxidant Potential of Ethyl Acetate Extract of Alstonia Boonei Leaves and Stem Bark
DOI:
https://doi.org/10.21580/wjc.v7i1.20667Keywords:
Alstonia boonei, Antioxidants potential, Mineral composition, Phytochemical, Antibacterial activityAbstract
Folkloric medicine has reported the effectiveness of Alstonia boonei stem bark and leaf extracts in treating and managing different ailments. Hence, this research is a comparative evaluation of the phytochemicals; antioxidant potential, mineral content, and antibacterial capacity of ethyl acetate extract of Alstonia boonei leaves and stem bark. Phytochemical analysis was determined using standard methods; mineral composition was determined using a flame photometer and an atomic absorption spectrophotometer; antibacterial capacity was analysed by agar-well diffusion method; and antioxidant potential was examined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. The phytochemicals detected were phenolics, eugenols, steroids, flavonoids, alkaloids, and reducing sugars in both extracts. Mineral content for sodium, iron, potassium, zinc, copper, magnesium and calcium was found to be 5.61 ± 1.33, 7.25 ± 1.00, 120.63 ± 1.39, 1.62 ± 0.30, 1.01 ± 0.01, 2.36 ± 0.10 and 15.61 ± 1.20 mg/kg respectively for the leaf extract, and 9.36 ± 0.35, 6.36 ± 0.55, 113.64 ± 0.45, 2.40 ± 0.32, 0.02 ± 0.00, 2.10 ± 0.50 and 28.30 ± 0.22 mg/kg respectively for the stem bark extract. The antioxidant potential at 250, 200, 150, 100, and 50 g/mL gave an IC50 of 2.89 ± 0.51, 3.90 ± 0.50, and 2.52 ± 0.22 μg/mL for leaf extract, stem bark extract and standard, respectively. The minimum inhibitory concentration (MIC) outcome was identical for both extracts except for P. aeruginosa. Both extracts' minimum bactericidal concentration (MBC) values were 12.5 to 50 mg/mL, except B. subtilis. Hence, this result provides valuable insight into the comparative usefulness of the plant in phytomedicine.
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References
Afolayan, A.J., and Meyer, J.J.M., (1997). The antimicrobial activity of 3, 5, 7 trihydroxyflavone isolated from the shoots of Helichrysum aureonitens. Journal of ethnopharmacology, 57(3), 177-181
Aghedo, O.N., and Ogbeide, O.N., (2022). Proximate Composition, Acute Toxicity and Antimicrobial Activity of Methanol Extract of Picralima nitida Stem Bark. ChemSearch Journal, 13(2), 92 – 98.
Alagbe, J. O., (2019). Proximate, Mineral and Phytochemical Analysis of Piliostigma Thonningii Stem Bark and Roots. International Journal of Biological, Physical and Chemical Studies, 1(1), 01–07
Iyasele, J.U., Uadia, J.O., Akhigbe, I.U., Jacob, J.N., and Ogbeide, O. K., (2022). Physico-Chemical Properties, Chemical Composition and Antimicrobial Activity of Adonidia merrillii Kernel Seed Oil. Trop J Nat Prod Res., 6(4), 599-605. doi.org/10.26538/tjnpr/v6i4.22
Kim, D.O., Jeong, S. W. and Lee, C.Y. (2003). Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem, 81, 321-326
Kokkaiah, I., Sethupandian, G., and Palanichamy, M., (2017). Antimicrobial activity of selected Indian Folk medicinal plants: Myristica fatua, Alstonia boonei, Helictress isora, Vitexaltissima and Atalantia racemosa. Asian Journal of Pharmaceutical and Clinical Research, 10(2), 277 – 280
Mollica, A., Zengin, G., Sinan, K.I., Marletta, M., Pieretti, S., Stefanucci, A., Etienne, O.K., Jek˝o, J., Cziáky, Z., and Bahadori, M.B., (2022). A Study on Chemical Characterization and Biological Abilities of Alstonia boonei Extracts Obtained by Different Techniques. Antioxidants, 11, 2171
Ogbeide, O.K., and Akhigbe, I.U., (2019). antihaemolytic, anti-anaemic and biosafety examination of combined telfairia occidentalis and ipomoea batatas leaves extract. Journal of Pharmaceutical and Allied Sciences, 16(4), 3106 – 3113.
Ogbeide, O.K., Aghedo, O.N., and Uadia, J.O. (2022). Anti-inflammatory and Analgesic Investigations of Methanol Extract of Ganodermalucidum. Trop J Phytochem Pharm Sci. 1(1), 17-22.
Ogbeide, O.K., Akhigbe, I.U., Unuigbe, C.A., Erharuyi, O., Imieje, V., Benjamin, G.O., & Falodun, A. (2023). Histological Assessment and Antimicrobial Investigation of Pure Compound; 3, 5, 6, 7-Tetrahydroxy-19-Vouacanoic Acid;(3β, 5α, 6β, 7β)-form, 6, 7-Dibenzoyl (Pulcherrimin A) Isolated from Caesalpinia pulcherrima Stem Bark. Tropical Journal of Phytochemistry and Pharmaceutical Sciences, 2(1), 30-37. https://doi. org/10.26538/tjpps/v2i1. 1.
Ogbeide, O.K., Akhigbe, I.U., Unuigbe, C.A., Erharuyi, O., Oseghale, I., Imieje, V., and Falodun, A., (2020). Isolation, characterisation and in vivo anti-malarial investigation of pulcherrimin A from Caesalpinia pulcherrima stem bark. GSC Biological and Pharmaceutical Sciences, 12(2), 056-063.
Ogbeide, O.K., Alao, E., and Jonathan, E., (2020). Phytochemical Investigation and Anti-Inflammatory Activity of Stem Bark of Pride of Barbados (Caesalpinia pulcherrima). Journal of Chemical Society of Nigeria, 45(3), 492-498.
Ogbeide, O.K., Dickson, V.O., Jebba, R.D., Owhiroro, D.A., Olaoluwa, M.O, Imieje V.O., Erharuyi, O., Owolabi B.J., Fasinu P., and Falodun, A., (2018). Antiplasmodial and acute toxicity studies of fractions and cassane-type diterpenoids from the stem bark of Caesalpinia pulcherrima (L.) Sw. Trop J Nat Prod Res., 2(4), 179-184.
Ogbeide, O.K., Ogbonnaya, C.J., Asakitikpi, E., Uyi, D.O., Aluge, B.O., Nosakhare, O., Otahagwa, W.O., Ehigie, C.A., Omoruyi, U., Aifuwa, O.S., and Gabriel, B.O., (2019). Analgesic and Anti-inflammatory Activities of the Stem Bark of Yellow Flamboyant (Peltophorum pterocarpum). J. Appl. Sci. Environ. Manage., 23(7), 1315-1322
Ogbeide, O. K., Omono, E. D., Ehizojie, P. O., Aiwonegbe, A. E., and Uadia, J.O., (2022). Phytochemical Investigation, Anti-inflammatory and Analgesic Activities of Ethyl Acetate Extract of Pride of Barbados Pod (Caesalpinia pulcherrima). Tanzania Journal of Science, 48(3), 548-558. https://dx.doi.org/10.4314/tjs.v48i3.3
Okorie, N.H., Mbah, C.J., Ugodi, G.W., and Magbo, C.C., (2022). Investigation into the Gastro-protective Properties of Alstoni aboonei (De Wild) Leaves and Stem Bark. Tropical Journal of Natural Product Research, 6(3), 403-407 https://dx.doi.org/10.26538/tjnpr/v6i3.17
Okoye, N.N., Nwokoye, J., and Okoye, C., (2022). Alstonia boonei De Wild (Apocynaceae) – A review of its Ethnomedicinal Uses, Phytochemistry and Pharmacological Activities. Journal of Current Biomedical Research, 1(4), 95–116.
Olanlokun, J.O., Fisayo, A.O., Oyebode, T.I., Clement, A., Moses, O.D., Mahmoud, S., and Olufunso, O.O., (2020). In vitro, in silico studies of newly isolated tetrahydro-4-(7-hydroxy-10-methoxy-6, 14-dimethyl-15-m-tolylpentadec-13-enyl) pyran-2-one and isobutyryl acetate compounds from Alstonia boonei stem bark. Journal of Molecular Structure, 12(16), 128225
Oluwafemi, K.A., Jesumoroti, O. J., Tinubu, B.E., and Uadia, J.O. (2015). Antioxidant Activities, Total Flavonoid and Total Phenolic Contents of Whole Plant of Kyllinga Erecta Shumach. Journal of Food and Nutrition Research, 3(8), 489-494. https://dx.doi.org/10.12691/jfnr-3-8-3.
Omoya, F., and Oyebola, T.F., (2019). Antiplasmodial activity of stem bark and leaves of Alstonia boonei De Wild. J of Microbiol and Exper., 7(5), 241‒ 245.
Opoku, F., and Akoto, O., (2014). Antimicrobial and Phytochemical Properties of Alstonia Boonei Extracts. Organic Chem Curr Res, 1, 137. https://dx.doi.org/10.4172/2161-0401.1000137.
Oppong, B.E., Dodoo, K. B., Kitcher, C., Gordon, A., Frimpong-Manso, S., and Schwinger, G., (2020). Pharmacognostic characteristics and mutagenic studies of Alstonia boonei De Wild. Res J Pharmacogn, 7(1), 7-15.
Trease, G.E., and Evans, W.C., (2002). Phytochemicals. In: Pharmacognosy, Saunders Publishers, London, p214
Uadia, J.O., Nnamdi, E.K., Chigozie, N., Ndubisi, V.I., and Ogbeide, O.K., (2023). Phytochemica Investigation, Proximate Composition, Acute Toxicity, Anti-Inflammatory and Antinociceptive Activities of Extracts of Caesalpinia Pulcherrima Linn Flower. Walisongo Journal of Chemistry, 6(2), 194-207. https://doi.org/10.21580/wjc.v6i2.18175
Uzor, P., Osadebe, P., Ozumba, B., Okafor, S., Eze, F., Odoh, U., and Onuoha, J., (2017) Oxytocic Effect of Extracts and Fractions of Alstonia boonei Stem Bark. Paper presented at the 65th International Conference of the Society for Medicinal Plants and Natural Product Research. Sept 3-7, Basel, Switzerland
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