Chromatography Profiles of Terpenoid Compounds in The Extract of Sambiloto (Andrographis paniculata) Herb from Various Solvents
DOI:
https://doi.org/10.21580/wjc.v4i2.7783Keywords:
Extraction solvent, Chromatographic profiles, Terpenoid, Sambiloto herbAbstract
Sambiloto (Andrographis paniculata) is widely used as a medicinal plant for Indonesians. The content of extracted bioactive compounds is affected by the polarity of the extraction solvent. This study aims to determine the effect of extraction solvent on the chromatographic profile of Sambiloto herb, especially terpenoid group using the TLC-Spectrophotodensitometry method. Sambiloto herb was extracted by sonication method using methanol, ethanol, ethyl acetate, and chloroform as the solvents. Chromatographic profiles were identified by stationary phase of TLC silica gel GF254 plate and a mobile phase of chloroform:methanol (10:1 v/v), observed visually, and scanned at 210 nm. The plate was sprayed with anisadehyd-sulfuric acid reagent and observed visually. The compounds that belong to terpenoid compounds, namely S2, S5, S8, S11, and S12, had a greater percentage of peak area than the total area so that they were categorized as major components. The extraction solvent for Sambiloto herb that provides the best chromatographic profiles was found in the solvent of methanol extraction.Downloads
References
Astarina, N. W. G., Astuti, K. W., Warditiani, N. K. (2012) ‘Skrining Fitokimia Ekstrak Metanol Rimpang Bangle (Zingiber purpureum Roxb.)’, Jurnal Farmasi Udayana, 344(4), pp. 1–7.
Avanigadda, S. and Vangalapati, M. (2010) ‘Experimental and Modelling Studies of Andrographolide Extraction from Andrographis paniculata’, International Journal of Chemical, Environmental and Pharmaceutical Research, 1(1), pp. 32–36.
Cui, Y. et al. (2009) ‘Fingerprint Profile of Active Components for Andrographis paniculata Nees by HPLC-DAD’, Sensing and Instrumentation for Food Quality and Safety, 3(3), pp. 165–179.
El Houda Lezoul, N. et al. (2020) ‘Extraction Processes with Several Solvents on Total Bioactive Compounds in Different Organs of Three Medicinal Plants’, Molecules, 25(20), pp. 1–15.
Kumoro, A. C. and Hasan, M. (2006) ‘Experimental and Modelling Studies of Andrographolide Extraction from Andrographis paniculata’, in University of Malaya ShortTerm Research Dissemnination. University of Malaya, pp. 1–4.
Li, S. et al. (2008) ‘Chemical Markers for The Quality Control of Herbal Medicines: An Overview’, Chinese Medicine, 3(7), pp. 1–16.
Rafi, M. et al. (2020) ‘Classification of Andrographis paniculata Extracts by Solvent Extraction using HPLC fingerprint and Chemometric Analysis’, BMC Research Notes. BioMed Central, 13(1), pp. 1–6.
Samirana, P. O. et al. (2017) ‘Penentuan Profil Kandungan Kimia Ekstrak Etanol Daun Binahong (Anredera scandens (L.) Moq.)’, Jurnal Farmasi Udayana, 6(1), pp. 23–33.
Schibli, A. and Reich, E. (2005) ‘Modern TLC: A Key Technique for Identification and Quality Control of Botanicals and Dietary Supplements’, Journal of Planar Chromatography - Modern TLC, 18(1), pp. 34-38.
Shakila, R., Elankani, P. and Jegajothipandian, S. (2014) ‘Development of Fingerprint Profiles for Androgrphis echioides Nees. and Andrographis paniculata Nees.’, Research Journal of Pharmacognosy and Phytochemistry, 6(1), pp. 22–29.
Sharma, M. and Sharma, R. G. (2013) ‘Identification, Purification and Quantification of Andrographolide from Andrographis paniculata (Burm. F.) Nees by HPTLC at Different Stages of Life Cycle of Crop.’, Journal of Current Chemical & Pharmaceutical Sciences, 3(1), pp. 23–32.
Sultana, B., Anwar, F. and Ashraf, M. (2009) ‘Effect of Extraction Solvent/Technique on the Antioxidant Activity of Selected Medicinal Plant Extracts’, Molecules, 14(6), pp. 2167–2180.
Truong, D. H. et al. (2019) ‘Evaluation of The Use of Different Solvents for Phytochemical Constituents, Antioxidants, and In Vitro Anti-Inflammatory Activities of Severinia buxifolia’, Journal of Food Quality, 2019(1), pp. 1–9.
Wagner, H. and Bladt, S. (2001) Plant Drugs Analysis: a Thin Layer Chromatography Atlas. 2nd Edition. Germany: Springer-Verlag Berlin Heidelberg.
Wang, S. et al. (2007) ‘Fingerprint Quality Control of Angelica sinensis (Oliv.) Diels by High-Performance Liquid Chromatography Coupled with Discriminant Analysis’, Talanta, 72(2), pp. 434–436..
Widjajakusuma, E. C. et al. (2019) ‘Phytochemical Screening and Preliminary Clinical Trials of The Aqueous Extract Mixture of Andrographis paniculata (Burm. f.) Wall. ex Nees and Syzygium polyanthum (Wight.) Walp Leaves in Metformin Treated Patients with Type 2 Diabetes’, Phytomedicine. Elsevier GmbH, 55(1), pp. 137–147.
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