Metabolit Sekunder dari Kulit Batang Kalesi (Sphatolobus Ferrogenus) Serta Uji Inhibitor Enzim A-Glukosinade

Moch Abdussalam  -  Universitas Yarsi, Indonesia
Indah Permata Yuda  -  Universitas Yarsi, Indonesia
Juniarti Juniarti*  -  Universitas Yarsi, Indonesia

(*) Corresponding Author

Tanaman herbal merupakan warisan kearifan lokal dalam upaya pengobatan dan mempertahankan kesehatan masyarakat. Secara tradisional masyarakat Kalimantan tengah menggunakan tanaman aka kalesi (Sphatolobus ferrogenus) sebagai obat-obatan tradisional yang cukup luas. Penelitian ini bertujuan untuk mengisolasi senyawa metabolit sekunder yang terdapat dalam ekstrak metanol kulit batang aka kalesi (Sphatolobus ferrogenus). Isolasi dilakukan dengan tahapan ekstraksi, fraksinansi dan purifikasi. Hasil penelitian didapatkan dua isolat yang telah diuji kemurniannya dengan metode kromatografi lapis tipis. Isolat diidentifikasi dengan spektroskopi 1H-NMR dan 13C-NMR didapatkan senyawa gliseril monostearate dan sigmasterol. Kedua senyawa diuji sifat antidiabetes dengan metode inhibitor enzim α-glukosidase. Aktivitas senyawa gliseril monostearate tidak aktif, sementara sigmasterol menunjukan aktivitas yang lemah dengan IC50= 388 ppm.

Keywords: gliseril monostearate; sigmasterol; Sphatolobus ferrogenus

  1. Al-samydai, A. and Al-mamoori, F. (2018) ‘Anti – Diabetic Activity of Cinnamon : A Review’, (August).
  2. Arumugam, G., Manjula, P. and Paari, N. (2013) ‘A review: Anti diabetic medicinal plants used for diabetes mellitus’, Journal of Acute Disease, 2(3), pp. 196–200. doi: 10.1016/s2221-6189(13)60126-2.
  3. Bedekar, A., Shah, K. and Koffas, M. (2010) Natural Products for Type II Diabetes Treatment. 1st edn, Advances in Applied Microbiology. 1st edn. Elsevier Inc. doi: 10.1016/S0065-2164(10)71002-9.
  4. Cahyana, Y. and Adiyanti, T. (2021) ‘Flavonoids as Antidiabetic Agents’, Indonesian Journal of Chemistry, 21(2), pp. 512–526. doi: 10.22146/ijc.58439.
  5. Chama, M. A. et al. (2016) ‘Isolation, characterization, and anthelminthic activities of a novel dichapetalin and other constituents of Dichapetalum filicaule’, Pharmaceutical Biology, 54(7), pp. 1179–1188. doi: 10.3109/13880209.2015.1059861.
  6. Chaudhary, N. and Tyagi, N. (2018) ‘Diabetes mellitus: An Overview’, International Journal of Research and Development in Pharmacy & Life Sciences, 7(4), pp. 3030–3033. doi: 10.21276/ijrdpl.2278-0238.2018.7(4).3030-3033.
  7. Dewi, R. T., Tachibana, S. and Darmawan, A. (2014) ‘Effect on α-glucosidase inhibition and antioxidant activities of butyrolactone derivatives from Aspergillus terreus MC751’, Medicinal Chemistry Research, 23(1), pp. 454–460. doi: 10.1007/s00044-013-0659-4.
  8. Doni Eko Saputra, N. H. dan M. W. W. (2014) ‘T. Haryati, et al. , ALCHEMY jurnal penelitian kimia , vol. 10, no. 2, hal. 148-156’, 10(2), pp. 148–156.
  9. Dowarah, J. and Singh, V. P. (2020) ‘Anti-diabetic drugs recent approaches and advancements’, Bioorganic and Medicinal Chemistry, 28(5), p. 115263. doi: 10.1016/j.bmc.2019.115263.
  10. Early Febrinda, A. et al. (2013) ‘Kapasitas Antioksidan Dan Inhibitor Alfa Glukosidase Ekstrak Umbi Bawang Dayak’, Jurnal Teknologi dan Industri Pangan, 24(2), pp. 161–167. doi: 10.6066/jtip.2013.24.2.161.
  11. Jenie, U. A. (2014) Teknik Modern Spektroskopi NMR : Teori dan Aplikasi dalam Elusidasi Struktur Molekul Organik.
  12. Kunle, Oluyemisi Folashade1*, Egharevba, Henry Omoregie1 and Ahmadu, P. O. (2012) ‘Standardization of herbal medicines - A review’, International Journal of Biodiversity and Conservation, 4(3), pp. 101–112. doi: 10.5897/ijbc11.163.
  13. Lianza, M. et al. (2021) ‘a Preliminary Test Case’.
  14. Marlina, E. (2007) ‘Analisis Senyawa Metabolit Sekunder Dari Batang Spatholobus ferrugineus (Zoll & Moritzi) Benth Yang Berfungsi Sebagai Antioksidan’, Jurnal Penelitian Mipa, 1(1), pp. 23–29. Available at: http://www.siafif.com/kuliah/sukma/semester 8/SKRIPSI KAKAK TINGKAT/lumut222/kpm-des2007-1 (2).pdf.
  15. Okur, M. E., Karantas, I. D. and Siafaka, P. I. (2017) ‘Diabetes mellitus: A review on pathophysiology, current status of oral medications and future perspectives’, Acta Pharmaceutica Sciencia, 55(1), pp. 61–82. doi: 10.23893/1307-2080.APS.0555.
  16. Perla, V. and Jayanty, S. S. (2013) ‘Biguanide related compounds in traditional antidiabetic functional foods’, Food Chemistry, 138(2–3), pp. 1574–1580. doi: 10.1016/j.foodchem.2012.09.125.
  17. Punthakee, Z., Goldenberg, R. and Katz, P. (2018) ‘Definition, Classification and Diagnosis of Diabetes, Prediabetes and Metabolic Syndrome’, Canadian Journal of Diabetes, 42, pp. S10–S15. doi: 10.1016/j.jcjd.2017.10.003.
  18. Purwati, A. I., Yunianto, P. and Supriyono, A. (2017) ‘Validasi Metode RP-HPLC untuk Penentuan Kadar Andrografolid Sebagai Senyawa Penanda pada Campuran Esktrak’, Chimica et Natura Acta, 5(3), p. 101. doi: 10.24198/cna.v5.n3.16056.
  19. Putra, Y. and Samirana (2020) ‘Anredera scandens’, Jurnal Farmasi Udayana, 8(2), pp. 85–94.
  20. Qais, N., Jahan, S. and Shajib, M. S. (2018) ‘A review on anti-diabetic plants’, Dhaka University Journal of Pharmaceutical Sciences, 17(1), pp. 139–152. doi: 10.3329/dujps.v17i1.37130.
  21. Setyowati, F. M. (2003) ‘Interrelationship between Kubu trible people and plant resources at the Bukit Duabelas biosphere reserve, Jambi’, Biodiversitas Journal of Biological Diversity, 4(1), pp. 47–54. doi: 10.13057/biodiv/d040110.
  22. Shakeel, F. et al. (2014) ‘Measurement and correlation of solubility of bioactive compound silymarin in five different green solvents at 298.15 K to 333.15 K’, Journal of Molecular Liquids, 195, pp. 255–258. doi: 10.1016/j.molliq.2014.02.039.
  23. Takeuchi, K., Baskaran, K. and Arthanari, H. (2019) ‘Structure determination using solution NMR: Is it worth the effort?’, Journal of Magnetic Resonance, 306, pp. 195–201. doi: 10.1016/j.jmr.2019.07.045.
  24. Vladimir S. Kislik (2012) Solvent Extraction (Clasical and Novel Approach). First. Amsterdam: Elsevier The Boulevard.
  25. Yin, Z. et al. (2014) ‘α-Glucosidase inhibitors isolated from medicinal plants’, Food Science and Human Wellness, 3(3–4), pp. 136–174. doi: 10.1016/j.fshw.2014.11.003.

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