Phytochemical Test and Antioxidant Activity of Methanol Extract in Arabica Coffee Leaves by Using DPPH Method (1,1-Diphenyl-2-Picrylhydrazyl)
DOI:
https://doi.org/10.21580/wjc.v4i2.8032Keywords:
Arabica coffee, phytochemical, antioxidantAbstract
The use of coffee plants is more focused on the coffee beans as a brewing drink or as a food additive. Parts of the coffee plant, such as leaves, are considered waste and have not been properly utilized. This study aimed to determine the phytochemical content and antioxidant activity of the methanol extract in Arabica coffee (Coffea arabica) leaves. The method used in this study was the extraction and DPPH method (1,1-diphenyl-2-picrylhydrazyl). The results showed that the Arabica coffee leaves contained flavonoids, alkaloids, terpenoids, and phenolic compounds. The antioxidant activity test showed an IC50 value of 57.699 ppm with the strong antioxidant category.
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Ajhar, N.M., Meilani, D. 2020. Skrining fitokimia dan uji aktivitas antioksidan dari ekstrak etanol biji kopi arabika (Coffea arabica) yang tumbuh di daerah gayo dengan metode DPPH. Pharma Xplore, 5(1), 34-40. doi: 10.36805
Campa, C.L., Mondolot, A., Rakotondravao, A., Bidel, L.P.R., Gargadennec, A., Couturon, E., La Fisca. P., Rakotomalala, J.J., JayAllemand, C and Davis, A.P. 2012. A survey of mangiferin and hydroxycinnamic acid ester accumulation in coffee (Coffea) leaves: biological implications and uses. Annals of Botany, 110 (3), 595–613. doi: 10.1093/aob/mcs119
Campa, C and Petitvallet, A. 2017. Beneficial compounds from coffee leaves,” In Achieving sustainable cultivation of coffee, ed. P. Lashermes (Cambridge: Burleigh Dodds Science Publishing Limited).
Farah, Adriana. 2012. Coffee :Emerging Health Effects and Disease Prevention, First Edition. John Willey & Sons, Inc and Institute of Food Technologists (USA).Wile Blackwell Publising Ltd;
Hecimovic, I., Cvitanovic, A.B., Horzic, D and D. Komes. 2011. Comparative study of poliphenols and caffeine in different coffee varieties affected by degree of roasting. Food Chemistry. 129: 991–1000. doi: 10.1016/j.foodchem.2011.05.059
Jyotshna, Khare,P. And Shanker, K. 2016. “Mangiferin: A review of sources and interventions for biological activities.Biofactors. 42 (5),504–514. doi: 10.1002/biof.1308
Luczkiewicz, P., Kokotkiewicz, A., Dampc, A.,Luczkiewicz, M. 2014. Mangiferin: A promising therapeutic agent for rheumatoid arthritis treatment.Medical Hypotheses. 83 (5), 570–574. doi: 10.1016/j.mehy.2014.08.021
Mangiwa, S., Maryuni, A. E. 2019. Skrining Fitokimia dan Uji Antioksidan Ekstrak Biji Kopi Sangrai Jenis Arabika (Coffea arabica) Asal Wamena dan Moanemani, Papua.Jurnal Biologi Papua. 11(2), 103-109. doi: 10.31957/jbp.925.
Marliana, S. D., Suryanti,V dan Suyono. 2005. Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis Komponen Kimia Buah Labu Siam (Sechium edule Jacq. Swartz.) dalam Ekstrak Etanol. Biofarmasi. 3(1), 26-31.
Molyneux, P. 2004. The Use of the Stable Free Radical diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity. J. Sci Technol,: 26(2), 211-219.
Patay, E.B., Nemeth, T., Nemeth, T. S., Filep, R., Vlaseh, L., Papp,N. 2016. Histological and phytochemical studies of Coffea Benghalensis B. Heyne Ex Schult., compared with Coffea arabica L.Farmacia, 64 (1), 125–130.
Pristianan, D.Y., Susanti, S., Nurwantoro. 2017. Antioksidan dan Kadar Fenol Berbagai Ekstrak Daun Kopi (Coffea sp.): Potensi Aplikasi Bahan Alami untuk Fortifikasi Pangan. Jurnal Aplikasi Teknologi Pangan. 6 (2), 89-92. doi:10.17728/jatp.205
Rendon, M.Y., Salva, T.J.G., Bragagnolo, N. 2014. Impact of chemical changes on the sensory characteristics of coffee beans during storage. Food Chem, 147, 279-86. doi: 10.1016/j.foodchem.2013.09.123.
Sholichah, E.,Apriani, R., Desnilasari, D.,. Karim, M.A. 2019. Produk samping kulit kopi arabika dan robusta sebagai sumberpolifenol untuk antioksidan dan antibakteri.Jurnal industri hasil perkebuanan, 14 (2), 57-66.
Upadhyay, R and L. J. Mohan Rao. 2013. An outlook on chlorogenic acids-occurrence, chemistry, technology, and biological activities.Critical Reviews in Food Science and Nutrition, 53 (9), 968–984. doi: 10.1080/10408398.2011.576319
Vijayanand, S., Thomas, A. S. 2016. Screening of Michelia champacca and Muntingia calabura extracts for potential Bioactives.International Journal of Pharma Sciences and Research (IJPSR), 7 (6), 266-273 (2016).
Wardana, Andika Pramudya dan Tukiran. 2016. Skrining Fitokimia dan Aktivitas Antioksidan Ekstrak Kloroform Tumbuhan Gowok (Syzygium Polycephalum). Prosiding Seminar Nasional Kimia Unesa.
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