Molecular characterization of heavy metal tolerant bacteria from an agricultural soil

Olusolape Afolake Favour Ilusanya*    -  Olabisi Onabanjo University , Ago-Iwoye, Nigeria
Oluwasegun Irouoghene Olajumoke  -  Olabisi Onabanjo University, Ago-Iwoye,Ogun State, Nigeria
Oyindolapo Christianah Fogbonja  -  Olabisi Onabanjo University, Ago-Iwoye, Nigeria
Haneefat Olabimpe Egberongbe  -  Olabisi Onabanjo University, Ago-Iwoye, Nigeria
Funmilayo Mujidat Oyeyipo  -  Olabisi Onabanjo University, Ago-Iwoye, Nigeria
Reuben Yakubu  -  Olabisi Onabanjo University, Ago-Iwoye, Nigeria

(*) Corresponding Author

The aim of this study was to isolate and characterize heavy metal tolerant bacteria from an agricultural soil in Ago Iwoye, Ogun Stae, Nigeria. Bacteria were isolated from soil samples collected and screened for tolerance to different concentrations of lead, copper and cadmium. Heavy metal tolerant bacteria were characterized using phenotypic and molecular techniques. All the isolates were able to grow and tolerate different concentration of the heavy metals while four isolates identified as Bacillus cereus, Pseudomonas aeruginosa, Altantibacter hermannii and Enterobacter quasihormaechei exhibited high degree of tolerance to Pb, Cu and Cd with minimum inhibitory concentrations of 1500mg/l of lead,1000mg/l of Cu and 700mg/l of Cd. These results highlighted the high potential of these bacteria as bioremediation tools of heavy metal contaminated soil.

Keywords: Heavy metal, tolerance, bacteria, agricultural soil

Keywords: Heavy metal, tolerance, bacteria, agricultural soil.

  1. Alengebawy, A., Abdelkhalek, S. T., Qureshi, S. R., & Wang, M. Q. (2021). Heavy metals and pesticides toxicity in agricultural soil and plants: Ecological risks and human health implications. Toxics, 9(3), 42.
  2. Bakulski, K.M., Hu, H., Park, & S.K. (2020). Chapter 51: Lead, cadmium and Alzheimer’s disease. The neuroscience of Dementia, 2, 813-830.
  3. Bansod B, Kumar T, Thakur R, Rana S, & Singh I (2017) A review on various electrochemical techniques for heavy metal ions detection with different sensing platforms. BiosensBioelectron 94:443–455. https://doi.org/10.1016/j.bios.2017.03.031
  4. Bergey, D.H., & Holt, J.G., (1984). Bergey's Manual of Systematic Bacteriology, Baltimore: Williams and Wilkins.
  5. Bhende, R. S., Jhariya, U., Srivastava, S., Bombaywala, S., Das, S., & Dafale, N. A. (2022). Environmental distribution, metabolic fate, and degradation mechanism of chlorpyrifos: recent and future perspectives. Applied Biochemistry and Biotechnology, 1-35.
  6. Chatterjee, S., Gupta, P., & Sarker, S. K. (2023). Bioengineering bacteria for enhanced bioremediation of heavy metals: Recent advances and future perspectives. Trends in Biotechnology, 51(8), 827–842.
  7. Choudhary, S., & Sharma, K. P. (2017). Heavy metal detoxification and tolerance mechanisms in plants: Implications for phytoremediation. Environmental Reviews, 25(4), 398-414.
  8. Du, D., Sun, Y., & Liu, W. (2023). Diverse heavy metal-resistant bacteria and their potential bioremediation applications in contaminated environments. Frontiers in Microbiology, 14, 1858824.
  9. Forsyth, J.E., Islam, M.S., Parvez, S.M., Raqib, R., Rahman, M.S., Muehe, E.M., Fendorf, S., & Luby, S.P. (2018). Prevalence of elevated blood lead levels among pregnant women and sources of lead exposure in rural Bangladesh: A case control study. Environmental Research, 166, 1-9.
  10. Genchi, G., Sinicropi, M.S., Lauria, G., Carocci, A., & Catalano, A. (2020). The effects of cadmium toxicity. International Journal of Environmental Research and Public Health, 17(11), 3782.
  11. Huq, M.E., Fahad, S., Shao, Z., Sarven, M.S., Khan, I.A., Alam, M., Saeed, M., & Ulah, H. (2020). arsenic in groundwater environment in Bangladesh: Occurrence and mobilization. Journal of Environmental Management, 262, 110318.
  12. Kepel, B. J., Bodhi, W., & Tallei, T. E. (2021, November). Heavy metal (As, Cd, Cr, Hg, Pb) analysis and identification of heavy metal resistant bacteria in sediments from Manado Bay. In IOP Conference Series: Earth and Environmental Science (Vol. 926, No. 1, p. 012096).
  13. Khan, A., Khan, S., Khan, M. A., Qamar, Z., & Waqas, M. (2015). The uptake and bioaccumulation of heavy metals by food plants, their effects on plants nutrients, and associated health risk: a review. Environmental science and pollution research, 22, 13772-13799.
  14. Kumar, A., Prasad, R., & Rai, V. K. (2022). Bacterial survival strategies and responses under heavy metal stress: A comprehensive overview. Reviews in Environmental Science & Biotechnology, 21(3), 309–339.
  15. Li, Y., Yang, Y., Wu, X., Wu, Y., Wu, Y., Chen, W., & Wei, G. (2023). Isolation of a novel multiple-heavy metal resistant Lampropedia aestuarii GYF-1 and investigation of its bioremediation potential. Journal of Hazardous Materials, 462, 128452. https://doi.org/10.1016/j.jhazmat.2023.128452
  16. Li, Y., Zhang, J., & Zhang, X. (2019). Identification of heavy metal resistant bacteria from soil contaminated with electroplating wastewater. Huan jing ke xue Huanjing kexue, 40(8), 3603-3610.
  17. Li, Z., Yang, Y., Wu, J., Zhao, H., & Li, T. (2022). Metal tolerance in bacteria: Physiological and molecular responses. Environment International, 166, 107399. https://doi.org/10.1016/j.envint.2022.107399
  18. Mani, D., & Kumar, C. (2014). Biotechnological advances in bioremediation of heavy metals contaminated ecosystems: an overview with special reference to phytoremediation. International journal of environmental science and technology, 11, 843-872.
  19. Ndiaye, O., Diop, A., Diop, M., Aïdara, S., Tine, A., & Gueye, E. H. (2023). Co-occurrence of heavy metals and antibiotics resistance in bacteria isolated from metal-polluted soil. Environmental Science and Pollution Research, 30(14), 12849–12861. https://doi.org/10.1007/s11356-023-20701-5
  20. Pandey, V.C., Zhang, M., Wang, J., Alam, M., & Saeed, M. (2019). Heavy metal contamination: an alarming threat to environment and human health. Environmental Science and Pollution Research, 26(16), 15938-15939.
  21. Rahman, K., Menon, U., & V, S. (2022). Removal of cadmium by heavy metal–resistant bacteria isolated from Hussain Sagar Lake—Hyderabad. Biomass Conversion and Biorefinery, 1-11.
  22. Rehman, K., Fatima, F., Waheed, I., & Akash, M. S. H. (2018). Prevalence of exposure of heavy metals and their impact on health consequences. Journal of cellular biochemistry, 119(1), 157-184.
  23. Saha, P., Mazumdar, A., & Basak, P. (2020). Isolation and identification of heavy metal-resistant bacteria from contaminated soil and their potential for bioremediation. International Journal of Environmental Science and Technology, 17(1), 193-204.
  24. Sandeep, G., Vijayalatha, K. R., & Anitha, T. (2019). Heavy metals and its impact in vegetable crops. Int. J. Chem. Stud, 7(1), 1612-1621.
  25. Sarwar, T., Shahid, M., Natasha, Khalid, S., Shah, A. H., Ahmad, N., & Bakhat, H. F. (2020). Quantification and risk assessment of heavy metal build-up in soil–plant system after irrigation with untreated city wastewater in Vehari, Pakistan. Environmental geochemistry and health, 42, 4281-4297.
  26. Sharma, S., Kumar, M., & Arora, P. (2023). Microbial biofilms for the bioremediation of heavy metals: Mechanisms and applications. Current Pollution Reports, 9(1), 1–15.
  27. Verma, S., Bhatt, P., Verma, A., Mudila, H., Prasher, P., & Rene, E. R. (2021). Microbial technologies for heavy metal remediation: effect of process conditions and current practices. Clean Technologies and Environmental Policy, 1-23.

Open Access Copyright (c) 2024 Al-Hayat: Journal of Biology and Applied Biology
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
apps