Antihyperuricemia Potential in Ethanol Extract of Siamese Bamboo (Thyrsostachys siamensis) Leaves on Male White House Mice (Mus musculus L.)
DOI:
https://doi.org/10.21580/wjc.v5i2.10666Keywords:
Hyperuricemia, Mus musculus L., Siamese Bamboo, Thyrsostachys siamensis, Uric AcidAbstract
Hyperuricemia is commonly treated with the synthesis drug allopurinol. Allopurinol lowers uric acid by inhibiting the enzyme xanthine oxidase but provides side effects on the skin and kidneys. In addition, it can be overcome with secondary metabolite compounds in plants. This compound is found in bamboo leaves that have long been used by Chinese people for treatment. This study aims to find out the ability of siamese bamboo leaf extract (Thyrsostachys siamensis) in lowering the uric acid levels of white house mice. Fine siamese bamboo leaves are extracted using 70% ethanol and then evaporated with a rotary evaporator. Obtained a viscous extract with a yield of 55.42% which was further analyzed the phytochemical content and obtained that siamese bamboo leaf extract contains alkaloids, flavonoids, saponins, tannins, and triterpenoids. The ability to reduce uric acid levels was tested in male white house mice (Mus musculus L) with six treatment groups, namely group I (without treatment), group II (negative control), group III (positive control), group IV (extract 5 mg / 20 g WW), group V (extract 10 mg / 20 g WW, group VI (extract 20 mg / 20 g WW) which had previously been adapted in the test cage environment. Tests of the activity of siamese bamboo leaf ethanol extract (Thyrsostachys siamensis) against the reduction of white house mouse uric acid showed the best percentage reduction at an extract dose of 5 mg / 20 g BB of 65.85%. This value does not differ much and is comparable to the decrease by allopurinol 0.78 mg / 20 g BB of 58.97%. Therefore, siamese bamboo leaves (Thyrsostachys siamensis) proved effective in lowering uric acid levels. This proves that siamese bamboo leaves can lower the uric acid levels of white house mice and have the potential to be used as a treatment material for hyperuricemia and is expected to provide information on the use of bamboo plants (Thyrsostachys siamensis) as a lowering of uric acid levels. The results of this uric acid reduction test can be used as a reference to determine how to consume bamboo leaf extract (Thyrsostachys siamensis) for the treatment or prevention of gout.
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Aini, P. N., Faisal, & Marwan, D. W. (2020). Analisis Pengaruh Pemberian Ekstrak Bawang Merah (Allium cepa L) Terhadap Kadar Asam Urat Darah Pada Tikus Putih ( Rattus Norvegicus ) Jantan yang Diinduksi Kalium Oksonat. Journal UNJA, 8(2), 147–153.
Anggraito, Y. U., Susanti, R., Iswari, R. S., Yuniastuti, A., Lisdiana, L., Habibah, N. A., & Bintari, S. H. (2018). Metabolit Sekunder dari Tanaman: Aplikasi dan Produksi. FMIPA Universitas Negeri Semarang.
Apriansyah. (2011). Pengaruh Ekstrak Etanol Buah Sirsak (Annona muricata Linn) terhadap Penurunan Kadar Asam Urat pada Tikus Sprauge-Dawley Jantan [Bachelor Thesis]. Pakuan University.
Brunton, L. L., Lazo, J. S., & Parker, K. L. (2006). Goodman & Gilman. Las Bases Farmacológicas de La Terapéutica, 12.
Dwityanti, D., Dewanti, E., & Rachmania, R. A. (2021). Anti-hyperuricemia Effect of Water Fraction Cinnamon (Cinnamomum burmannii (Ness & T. Ness) Blume) on White Male Rats. SCITEPRESS, 102–106. https://doi.org/10.5220/0008240101020106
Fauzan, A., & Yuli Kusumawati, S. (2017). Hubungan Indeks Massa Tubuh (IMT), Asupan Purin dan Olahraga dengan Kejadian Gout Arthritis pada Lansia di Wilayah Kerja Puskesmas Tanjungsari Pacitan.
Fitrya, F., & Muharni, M. (2014). An Antihyperuricemia Effect of Ethanol Extract of Tunjuk Langit Rhizome (Helmynthostachys Zaylanica Linn Hook) on Swiss Male Mice. Traditional Medicine Journal, 19(1), 14–18.
Florencia, F. (2020). Minuman fermentasi ekstrak etil asetat kulit melinjo merah menggunakan bakteri asam laktat sebagai antihiperurisemia pada tikus wistar= Fermented drink of ethyl acetate extract red melinjo peel using lactic acid bacteria as anti-hyperurisemia on wistar rats.
Gunarti, N. S., Hidayah, H., Adzkia, A. H., & Mursal, I. L. P. (2021). Potensi Tanaman Tempuyung (Sonchus arvensis L.) Sebagai Antihiperurisemia Berdasarkan Kandungan Senyawa Aktif: Literature Review Article. Jurnal Buana Farma: Jurnal Ilmiah Farmasi, 1(2), 23–29.
Harborne, J. B. (2013). The Flavonoids: Advances in Research since 1980. Springer.
Hardian, H., Sulistiarini, R., & Rijai, L. (2014). Aktivitas Antihiperurisemia Ekstrak Etanol Daun Lada (Piper nigrum L.) pada Mencit (Mus musculus L.). Journal of Tropical Pharmacy and Chemistry, 2(5), 264–271.
Kamonwannasit, S., Rattamanee, C., & Kamcharoen, A. (2019). Phytochemical Screening, Total Phenolic Contents and Antioxidant Activity of the Aqueous Extracts of Dendrocalamus membranaceus and Thyrsostachys siamensis. Conference Proceedings The 6Th International Conference on Food Agriculture and Biotechnology, 6. https://doi.org/10.14457/MSU.res.2019.2
Khasanah, H. R., Meinisasti, R., & Tutut, A. R. (2019). Profil Kadar Asam Urat Pada Pengkonsumsi Minuman Tuak Di Singaran Pati Kota Bengkulu. Journal of Nursing and Public Health, 7(2), 13–18.
Klinjapo, R. (2020). Microencapsulation of Bamboo Leaves Extract from Pai Tong (Dendocalamus asper J.H. SCHUTTES) and Pai Ruak Pai Ruak (Thyrsostachys siamensis GAMBLE) and Their Antioxidant Properties. Assumption University of Thailand.
Kurniawati, E. (2017). Daya Antibakteri Ekstrak Etanol Tunas Bambu Apus Terhadap Bakteri Escherichia coli dan Staphylococcus aureus Secara In Vitro. Jurnal Wiyata: Penelitian Sains Dan Kesehatan, 2(2), 193–199.
Latief, M., Tarigan, I. L., Sari, P. M., & Aurora, F. E. (2021). Aktivitas Antihiperurisemia Ekstrak Etanol Daun Sungkai (Peronema canescens Jack) Pada Mencit Putih Jantan. Pharmacon: Jurnal Farmasi Indonesia, 18(1), 23–37.
Puspitaningrum, I., Kusmita, L., & Franyoto, Y. D. (2017). Aktivitas Imunomodulator Fraksi Etil Asetat Daun Som Jawa (Talinum triangulare (Jacq.) Willd) Terhadap Respon Imun Non Spesifik. Sekolah Tinggi Ilmu Farmasi “Yayasan Pharmasi Semarang,” 24–29.
Rasyad, A. A., Nurbaya, & Erjon. (2019). Effects of pineapple (Ananas comocus (L .) Merr) extract to lower uric acid levels in hyperurismic in male rats Pengaruh pemberian ekstrak nanas (Ananas comocus (L .) Merr) terhadap penurunan kadar asam urat pada tikus jantan hiperurisemia. Jurnal Ilmiah Farmasi, 15(2), 64–69.
Restina, Y., & Effendi, E. M. (2020). Efek Ekstrak Etanol 70% Herba Kemangi (Ocimum americanum L.) Sebagai Penurun Kadar Asam Urat Pada Tikus Jantan Galur Sprague Dawley. EKOLOGIA, 18(2), 49–54.
Rusman, A. D. P. (2021). Analisis Penyebab Faktor Resiko terhadap Peningkatan Penderita Gout (Asam Urat) di Wilayah Kerja Puskesmas Suppa Kecamatan Suppa Kabupaten Pinrang. Jurnal Ilmiah Manusia Dan Kesehatan, 4(1), 1–9.
Suhendi, A., Nurcahyanti, M., & Sutrisna, E. (2011). Aktivitas antihiperurisemia ekstrak air jinten hitam (Coleus ambonicus Lour) pada mencit jantan galur balb-c dan standardisasinya. Majalah Farmasi Indonesia, 22(2), 77–84.
Susanti, L. (2019). Ethanol Fraction of Corn Silk (Zea mays L.) to Decrease Uric Acid Levels in Male White Mice. JFL: Jurnal Farmasi Lampung, 8(2), 95–102.
SYFRIDIANA, R. (2017). Analisis Penghambatan Xanthine Oxidase Ekstrak Etanol Teh Hijau (Camelia sinensis) Menggunakan Metode Spektrofotometri UV-Vis.
Trevor, A. J., Katzung, B. G., Masters, S. B., & Kruidering-Hall, M. (2010). Pharmacology examination & board review. McGraw-Hill Medical New York, NY, USA:
Wahyuningsih, S., Sukandar, E. Y., & Sukrasno, L. D. (2016). Antihyperuricemia activity of the ethanol extract of Roselle calyx and its fraction (Hibiscus sabdariffa Linn) on male wistar rats. Int. J. Pharm. Pharm, Sci., 8, 278–280.
Wardatun, S., & Rahmi, I. (2012). Pengaruh Sari Buah Sirsak (Annona muricata Linn) Sebagai Penurun Kadar Asam Urat Tikus Sprague Dawley.
Zuraida, Z., Sulistiyani, S., Sajuthi, D., & Suparto, I. H. (2017). Fenol, Flavonoid, dan Aktivitas Antioksidan Pada Ekstrak Kulit Batang Pulai (Alstonia scholaris R.Br). Jurnal Penelitian Hasil Hutan, 35(3), Article 3. https://doi.org/10.20886/jphh.2017.35.3.211-219
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