EVALUATION OF PHOSPHOMOLYBDENUM AND FERRIC REDUCING  ANTIOXIDANT POWER ASSAYS IN EXTRACT COMBINATIONS OF  MANGIFERA INDICA AND EUPHORBIA HIRTA

I Made Wisnu Adhi Putra; Nyoman Suarjana

doi:10.21580/wjc.v8i1.25580

Authors

I Made Wisnu Adhi Putra Department of Biology, Faculty of Health and Science, Universitas Dhyana Pura, Badung, Bali, Indonesia
Nyoman Suarjana Department of Public Health, Faculty of Health and Science, Universitas Dhyana Pura, Badung, Bali, Indonesia

DOI:

https://doi.org/10.21580/wjc.v8i1.25580

Keywords:

free radicals, medicinal plants, oxidative stress, phytochemicals, synergistic effect

Abstract

Combining plant extracts at lower doses may enhance antioxidant effects through synergy. This
study investigated the antioxidant capacity of combined Mangifera indica and Euphorbia hirta leaf
extracts using metal ion-reducing power assays. The extracts were prepared via maceration with
70% ethanol. Phytochemical profiling was conducted through preliminary screening tests.
Antioxidant capacity was assessed spectrophotometrically using the phosphomolybdenum and
ferric reducing antioxidant power (FRAP) assays. The phosphomolybdenum assay involved
incubation of the extracts with reagent at 95°C for 90 minutes, while the FRAP assay was
conducted at 37°C for 15 minutes. Extract combinations were prepared in the ratios of 1:3, 1:1, and
3:1. Phytochemical screening revealed the presence of alkaloids, phenolics, flavonoids, steroids, and
terpenoids in both extracts. In the phosphomolybdenum assay, the Mo(VI) reducing power of M.
indica extract (29.154±0.664 mg AAE/g extract) was higher than that of E. hirta (27.948±0.667 mg
AAE/g extract). Similarly, in the FRAP assay, the ferric reducing power of M. indica extract
(55.304±1.284 mg AAE/g extract) exceeded that of E. hirta (48.009±1.873 mg AAE/g extract). The
highest reducing powers were observed in Comb. 3, yielding 30.745±0.715 mg AAE/g extract
(phosphomolybdenum) and 57.190±1.431 mg AAE/g extract (FRAP). Among the three
combinations tested, only the 3:1 ratio demonstrated a synergistic antioxidant effect in both assays.

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Author Biography

I Made Wisnu Adhi Putra, Department of Biology, Faculty of Health and Science, Universitas Dhyana Pura, Badung, Bali

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