PRELIMINARY PHYTOCHEMICAL ANALYSIS OF THE METHANOL EXTRACT OF RHODODENDRON RENSCHIANUM FLOWERS, ENDEMIC TO KELIMUTU NATIONAL PARK, INDONESIA, USING UV–VIS SPECTROPHOTOMETRY

Damiana Nofita Birhi; Faustina De Yesu Prisila Abi; Antonia Fransiska Laka; Ni Putu Vidya Primarista

doi:10.21580/wjc.v9i1.32005

Authors

Damiana Nofita Birhi Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Flores, East Nusa Tenggara, Indonesia, Indonesia
Faustina De Yesu Prisila Abi Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Katolik Widya Mandira, East Nusa Tenggara, Indonesia, Indonesia
Antonia Fransiska Laka Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Flores, East Nusa Tenggara, Indonesia, Indonesia
Ni Putu Vidya Primarista Department of Chemical Engineering, Politeknik Negeri Sriwijaya, South Sumatra, Indonesia, Indonesia

DOI:

https://doi.org/10.21580/wjc.v9i1.32005

Keywords:

Kelimutu lake, Phytochemical screening, Rhododendron renschianum, UV-Vis quantification

Abstract

Rhododendron renschianum is an endemic Indonesian species characterized by bell-shaped flowers with bright red petals and a yellowish base. This study aimed to document the presence of and quantify major phytochemical groups in the methanolic extract of R. renschianum flowers. Phytochemical screening yielded positive results for triterpenoids, tannins, flavonoids, alkaloids, and quinones. However, quantitative analysis using UV–Vis spectrophotometry indicated an apparent triterpenoid content of 2685.48 ± 123.49 mg UA/g, highlighting a limitation of UV–Vis analysis for investigating reactive compounds such as triterpenoids. The remaining compounds, in ascending order of content, were flavonoids, alkaloids, tannins, and quinones, with values of 5.98 ± 0.70 mg QE/g, 90.09 ± 67.09 mg CAF/g, 109.24 ± 1.44 mg TA/g, and 130.93 ± 30.19 mg HQ/g, respectively. These findings add to the baseline data for the initial investigation of the secondary metabolite content of this endemic species and provide a preliminary basis for further pharmacological research.

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Author Biographies

Damiana Nofita Birhi, Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Flores, East Nusa Tenggara, Indonesia

Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Flores, East Nusa Tenggara, Indonesia

Faustina De Yesu Prisila Abi, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Katolik Widya Mandira, East Nusa Tenggara, Indonesia

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Katolik Widya Mandira, East Nusa Tenggara, Indonesia

Antonia Fransiska Laka, Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Flores, East Nusa Tenggara, Indonesia

Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Flores, East Nusa Tenggara, Indonesia

Ni Putu Vidya Primarista, Department of Chemical Engineering, Politeknik Negeri Sriwijaya, South Sumatra, Indonesia

Department of Chemical Engineering, Politeknik Negeri Sriwijaya, South Sumatra, Indonesia

References

[1] Jia, S., Wang, Y., Hu, J., Ding, Z., Liang, Q., Zhang, Y., and Wang, H., 2016, Mineral and metabolic profiles in tea leaves and flowers during flower development, Plant Physiol. Biochem., 106, 316–326.

[2] Kanani, M., Chamani, E., Shokouhian, A. A., and Giglou, M. T., 2021, Plant secondary metabolism and flower color changes in damask rose at different flowering development stages, Acta Physiol. Plant., 43 (4), 1–10.

[3] Yeloojeh, K. A., Saeidi, G., and Sabzalian, M. R., 2020, Drought stress improves the composition of secondary metabolites in safflower fl ower at the expense of reduction in seed yield and oil content, Ind. Crop. Prod., 154 (June), 112496.

[4] Jain, C., Khatana, S., and Vijayvergia, R., 2019, Bioactivity of Secondary Metabolites of Various Plants: A Review, Int. J. Pharm. Sci. Res., 10 (2), 494–504.

[5] Bosco, N. S., and Butnariu, M., 2022, The biological role of primary and secondary plants metabolites, J. Nutr. Food Process., 5 (3), 1–7.

[6] Onder, S., Tonguc, M., Erbas, S., Onder, D., and Mutlucan, M., 2022, Investigation of phenological , primary and secondary metabolites changes during flower developmental of Rosa damascena, Plant Physiol. Biochem., 192 (October), 20–34.

[7] Park, C. H., Yeo, H. J., Kim, N. S., Park, Y. E., Park, S., Kim, J. K., and Park, S. U., 2018, Metabolomic Profiling of the White, Violet, and Red Flowers of Rhododendron schlippenbachii Maxim., Molecules, 23 (827), 1–11.

[8] Park, C. H., Yeo, H. J., Kim, Y. J., Nguyen, B. Van, Park, Y. E., Sathasivam, R., Kim, J. K., and Park, S. U., 2021, Profiles of Secondary Metabolites ( Phenolic Acids , Carotenoids , Anthocyanins , and Galantamine ) and Primary Metabolites ( Carbohydrates , Amino Acids , and Organic Acids ) during Flower Development in Lycoris radiata, Biomolecules, 11 (248), 1–14.

[9] Yang, L., Han, X., Liu, H., Chen, Y., Wu, M., and Tan, L., 2025, Effects of different flowering stages on secondary metabolites in Bletilla striata fl owers analyzed by GC-MS and LC-MS, Front. Ecol. Evol., 13 (October), 1–18.

[10] Lopez, N. M., Bagur, J. M., Lorenzo, Candida, Navarro, M. E. M., Salinas, M. R., and Alonso, G. L., 2019, Bioactivity and Bioavailability of the Major Metabolites of Crocus sativus L. Flower, Molecules, 24 (2827), 1–24.

[11] Mohsen, E., Younis, I. Y., and Farag, M. A., 2020, Industrial Crops & Products Metabolites profiling of Egyptian Rosa damascena Mill . flowers as analyzed via ultra-high-performance liquid chromatography-mass spectrometry and solid-phase microextraction gas chromatography-mass spectrometry in relation to , Ind. Crop. Prod., 155 (March), 112818.

[12] Grimbs, A., Shrestha, A., Rezk, A. S. D., Grimbs, S., Said, I. H., Schepker, H., Hütt, M., Albach, D. C., Brix, K., Kuhnert, N., and Ullrich, M. S., 2017, Bioactivity in Rhododendron : A Systemic Analysis of Antimicrobial and Cytotoxic Activities and Their Phylogenetic and Phytochemical Origins, Front. Plant Sci., 8 (April), 1–16.

[13] Wen, S., Cai, X., Zhou, K., Min, Y., Shang, C., Shen, L., Deng, L., Liu, D., Qiao, G., and Shen, X., 2025, Metabolome and comparative genome provide insights into secondary metabolites generation of a rare karst-growing Rhododendron in vitro culture, Plant J., 1–23.

[14] He, J., Shang, X., Dai, L., Yang, X., Li, B., Wei, Y., Zhang, J., and Pan, H., 2022, Chemical constituents , antibacterial , acaricidal and anti-inflammatory activities of the essential oils from four Rhododendron species, Front. Vet. Sci., 9, 1–12.

[15] Vengryt, M., and Raudone, L., 2024, Phytochemical Profiling and Biological Activities of Rhododendron Subsect . Ledum : Discovering the Medicinal Potential of Labrador Tea Species in the Northern Hemisphere, Plants, 13 (901), 1–15.

[16] Zhou, G., and Zhu, P., 2020, De novo transcriptome sequencing of Rhododendron molle and identification of genes involved in the biosynthesis of secondary metabolites, BMC Plant Biol., 20 (414), 1–19.

[17] Lema, Y., and Dewi, N. K., 2024, Development of Biodiversity E-Module in Kelimutu National Park Area to Improv Learning Outcomes of High School Students, J. Biol. Educ., 2024 (12), 1–8.

[18] Birhi, D. N., Kusuma, M. T., and Laka, A. F., 2025, Study of Indonesian Rhododendron : Classification, Conservation, and Pharmacology Activity, Eksakta Berk. Ilm. Bid. MIPA, 26 (02), 140–162.

[19] Sare, F. L. L., 2009, Pusat Penelitian Dan Pelestarian Taman Nasional Kelimutu Di Kab. Ende, Flores, Nusa Tenggara Timur, Atma Jaya Jogyakarta.

[20] Shalihi, D. J., Murniati, N., Rahman, W., Hutabarat, P. W. K., and Lailaty, I. Q., 2025, Plant Morphology, Paradermal Anatomy, and Leaves Metabolite Profiles of Rhododendron multicolor Miq. from Cibodas Botanic Garden, West Java, Indonesia, J. Smart Bioprospecting Technol., 06 (04), 32–51.

[21] Mambrasar, Y. M., Warseno, T., Kuswantoro, F., and Arifiani, D., 2024, Notes on Rhododendron (Ericaceae) From Bali, Indonesia, and A New Subspecies of Rhododendron Javanicum, Edinburgh J. Bot., 11, 1–11.

[22] Putri, D. M. S., 2011, Fenologi Rhododendron spp . (Subgenus Vireya) Koleksi Kebun Raya Eka Karya Bali, J. Hort, 21 (3), 232–244.

[23] Appolloni, E., Pennisi, G., Zauli, I., Carotti, L., Paucek, I., Quaini, S., and Gianquinto, G., 2021, Beyond vegetables : effects of indoor LED light on specialized metabolite biosynthesis in medicinal and aromatic plants , edible fl owers , and microgreens, J. Sci. Food Agric., (September), 472–487.

[24] Sleumer, 2026, Rhododendron Renschianum Sleumer. Available at: https://www.gbif.org/species/4166751 (Accessed: May 3, 2026).

[25] Hayat, J., Akodad, M., Moumen, A., Baghour, M., Skalli, A., Ezrari, S., and Belmalha, S., 2020, Heliyon Phytochemical screening , polyphenols , fl avonoids and tannin content , antioxidant activities and FTIR characterization of Marrubium vulgare L . from 2 different localities of Northeast of Morocco, Heliyon, 6 (September), 1–9.

[26] Rao, A., Kumari, S., Laura, J. S., and Dhania, G., 2023, Qualitative Phytochemical Screening of Medicinal Plants Using Different Solvent Extracts, Orient. J. Chem., 39 (3), 621–626.

[27] Çilesizoğlu, N. B., Yalçin, E., Çavuşoğlu, K., and Sipahi Kuloğlu, S., 2022, Qualitative and quantitative phytochemical screening of Nerium oleander L. extracts associated with toxicity profile, Sci. Rep., 12 (1), 1–16.

[28] Bharathy, P., Thanikachalam, P. V, Shoban, A. N., and Himayavendhan, H. V, 2024, Floral Fusion : Unravelling the Potent Blend of Ixora coccinea and Rhododendron arboreum for Health and Safety Benefits, Cureus, 16 (9), 1–15.

[29] Muthukumaran, P., Saraswathy, N., Aswitha, V., Balan, R., Gokhul, V. B., Indumathi, P., and Yuvapriya, S., 2016, Assessment of total phenolic , flavonoid , tannin content and phytochemical screening of leaf and flower extracts from Peltophorum pterocarpum ( DC .) Backer ex K . Heyne : a comparative study, Pharmacogn. J.J., 8 (2), 140–143.

[30] Shahdeo, P., and Kumar, A., 2024, Phytochemical and Antioxidant Evaluation of Cymbopogon Citratus Plant Grown in Ranchi District of Jharkhand, World J. Pharm. Res., 13 (13), 1638–1651.

[31] Taleuzzaman, M., 2018, Limit of Blank (LOB), Limit of Detection (LOD), and Limit of Quantification (LOQ), Org. Med. Chem. Int. J., 7 (5), .

[32] Rifai, B., Ihsan, P., Nurhayati, I. P., and Maysaroh, I., 2018, Method Validation of Ultra High Performance Chromatography-Double Mass Spectrometry ( UHPLC-MS / MS ) for Analysis of Curcumin in Ethanol Extract of Turmeric ( Curcuma longa ) with Various Comparisons, Pharm. J. Indones., 4 (1), 29–34.

[33] Khatri, D., Poudel, A., Ray, M. K., Gupta, N. K., Kunwar, S., Parajuli, S., Purja, S., Adhikari, S., Adhikari, R., Bahadur, S., Chhetri, B., and Poudel, P., 2025, Phytochemical Profiling and Antioxidant Potential of Three Rhododendron Species Collected from Mustang District, Nepal, Himalaya Biodivers., 10 (May), 100–109.

[34] Kom, Y. D., Ramaswamy, K., and Suresh, S., 2024, Phytochemical profiling and antioxidant evaluation of Rhododendron arboreum Sm leaf and flower : integrative analysis using advanced analytical techniques, Drug Dev. Ind. Pharm., 50 (7), 687–705.

[35] Mardina, V., Halimatussakdiah, Harmawan, T., Ilyas, S., Tanjung, M., Aulya, W., and Nasution, A., 2020, Preliminary phytochemical screening of different solvent extracts of flower and whole plant of Wedelia biflora, IOP Conf. Ser. Mater. Sci. Eng., 725, 1–6.

[36] Vala, M., and Maitreya, B., 2019, Qualitative analysis , total phenol content ( TPC ) and total tannin content ( TTC ) by using different solvant for flower of Butea monosperma ( Lam .) Taub . collected from Saurashtra region, J. Pharmacognsy Phytochem., 8 (3), 2902–2906.

[37] Kashyap, P., and Anand, S., 2016, Phytochemical and GC-MS analysis of Rhododendron arboreum flowers, Int. J. Farm Sci., 6 (4), 145–151.

[38] Khafid, A., Wiraputra, M. D., Putra, A. C., Khoirunnisa, N., Putri, A. A. K., Suedy, S. W. A., and Nurchayati, Y., 2023, Uji Kualitatif Metabolit Sekunder pada Beberapa Tanaman yang Berkhasiat sebagai Obat Tradisional, Bul. Anat. dan Fisiol., 8 (1), 61–70.

[39] Faizal, A., and Geelen, D., 2013, Saponins and their role in biological processes in plants, Phytochem. Rev., 12 (4), 877–893.

[40] Emilia, I., Setiawan, A. A., Novianti, D., Mutiara, D., and Rangga, 2023, Skrining Fitokimia Ekstrak Daun Sungkai (Peronema canescens Jack.) Secara Infundasi dan Maserasi, J. Indobiosains, 5 (2), 95–102.

[41] Buta, M. B., 2021, Effects of High Hydristatic Pressure (HHP) and Conventional Food Processing Techniques on The Nutritional Quality of Grass Pea Seeds/Products, .

[42] Safdar, A., Iftikhar, S., Karim, M., Khan, R., Hayat, M. Q., Afsar, T., Almajwal, A., Amor, H., and Razak, S., 2025, Rapid and cost-effective colorimetric detection of alkaloids in botanical extracts using a novel protocol with compressed tablets, Sci. Rep., 15 (26332), 1–15.

[43] BenchChem Technical Support Team, 2026, A Comparative Guide to Alkaloid Detection Reagents : Cobalt ( II ) Thiocyanate vs . Traditional Precipitating Agents. .

[44] Taranto, F., Pasqualone, A., Mangini, G., Tripodi, P., Miazzi, M. M., Pavan, S., and Montemurro, C., 2017, Polyphenol Oxidases in Crops : Biochemical , Physiological and Genetic Aspects, Int. J. Mol. Sci., 18 (377), 1–16.

[45] Yu, W., Gong, F., Xu, H., and Zhou, X., 2024, Molecular Mechanism of Exogenous ABA to Enhance UV-B Resistance in Rhododendron chrysanthum Pall. by Modulating Flavonoid Accumulation, Int. J. Mol. Sci., 25 (5248), 1–25.

[46] Ahmad, M. P., Ali, S., Wahab, S., Perween, T., Shahzad, N., and Dhami, D. B., 2022, Traditional uses, Pharmacology and Phytochemistry of National flower of Nepal Rhododendron arboreum: A Review, J. Drug Deliv. Ther., 12 (4), 162–166.

[47] Han, M., and Kim, K. Y., 2021, Research Progress on the Identification and Pharmacological Activity of the Active Components of the Rhododendron Species, Biosci. Biotechnol. Res. Asia, 18 (September), 543–565.

[48] Luitel, A., Shrestha, R., Pant, L. M., Shrestha, A. K., Thapa, N., Shrestha, M., Shresta, R., Hullatti, K., and Lamichhane, J., 2026, Bioactivity assessment of methanolic extract of Rhododendron cowanianum: An endemic species of Nepal, J. Adv. Pharm. Technol. Res., 17 (1), 44–50.

[49] Łyko, L., Nowak, R., Olech, M., Gawlik, U., Krajewska, A., Kalemba, D., and Prokopiv, A., 2025, Chemical profile of Rhododendron luteum Sweet leaf supercritical CO2 extracts and their anti-inflammatory and antidiabetic potential, Front. Chem., 13 (April), 1–13.

[50] Shalihi, D. J., Murniati, N., Rahman, Wiguna, Hutabara, P. W. K., and Lailaty, I. Q., 2025, Plant Morphology, Paradermal Anatomy, and Leaves Metabolite Profile of Rhododendron multicolor Miq. from Cibodas Botanic Garden, West Java, Indonesia, JSMARTech, 06 (04), .

[51] Shyaula, S. L., Choudhary, M. I., Musharraf, S. G., Joshi, B., Maharjan, R., and Ali, A., 2024, Identification of Anthocyanin , Flavonoids , Triterpenoids and Phosphatidylcholines from Rhododendron arboreum by Using LC-ESI-MS / MS Analysis, Mass Spectrom. Lett., 15 (3), 141–148.

[52] Nurani, L. H., Edityaningrum, C. A., Irnawati, I., Putri, A. R., Windarsih, A., Guntarti, A., and Rohman, A., 2023, Review: Chemometrics-Assisted UV-Vis Spectrophotometry for Quality Control of Pharmaceuticals: A Review, Indones. J. Chem., 23 (2), 542–567.

[53] Haque, M. M., Sultana, N., Abedin, S. M. T., and Kabir, S. E., 2019, Phytochemical screening and determination of minerals and heavy metals in the flowers of Nyctanthes arbor-tristis L ., Bangladesh J. Sci. Ind. Res., 54 (4), 321–328.

[54] Dai, J., Wang, X., Meng, X., Zhang, X., Zhou, Q., Zhang, Z., Zhang, X., Yi, Y., Liu, L., and Shen, T., 2023, UPLC – MS / MS and Gene Expression Research to Distinguish the Colour Differences of Rhododendron liliiflorum H . Lév, Horticulturae, 9 (1351), 1–16.

[55] Xia, X., Gong, R., and Zhang, C., 2022, Integrative analysis of transcriptome and metabolome reveals flavonoid biosynthesis regulation in Rhododendron pulchrum petals, BMC Plant Biol., 22 (401), 1–20.

[56] Aribowo, A. I., Lubis, C. F., Urbaningrum, L. M., Rahmawati, N. D., and Anggraini, S., 2021, Isolasi dan Identifikasi Senyawa Flavonoid Pada Tanaman, J. Heal. Sains, 2 (6), 751–757.

[57] Postolache, A. N., Velescu, I. D., Stoica, F., Crivei, I. C., Arsenoaia, V. N., Usturoi, M. G., Constantinescu (Pop), C. G., Lipsa, F. D., Frunza, G., Simeanu, D., and Ratu, R. N., 2023, A Clean-Label Formulation of Fortified Yogurt Based on Rhododendron Flower Powder as a Functional Ingredient, Foods, 12 (4365), 1–18.