Anti Bacterial Activity of Fungi TIF-1 as Saponin Producer

Willy Tirza Eden, Ersanghono Kusumo, Eko Budi Susatyo, Kasmui Kasmui


Treatment of microbial resistance is one of humankind's greatest challenges in terms of fight against infectious diseases. One way to overcome microbial resistance is discovery of potent new antimicrobial compounds against variety of microbial pathogens. Land is one potential source of microorganisms. This study aims to screen in order to get a fungal strains isolated from soil exposed to waste the pharmaceutical industry which possess antibacterial activity. Antibacterial activity test conducted on two types of bacteria, namely Staphylococcus aureus as Gram-positive bacteria and Escherichia coli as Gram-negative bacteria. The test results can be known isolates of fungi which has potential as an antibacterial, for then these are fermented for 10 days. Fermented then partitioned by using ethyl acetate. Initial screening showed that the fungi code TIF-1 as the most active fungi. Ethyl acetate soluble fraction was then identified the active compounds using thin layer chromatography method with various reagent spray. Bioautografi test results show that the saponins which play role in the antibacterial activity. ©2016 JNSMR UIN Walisongo. All rights reserved.


Antibacterial Agent; Soil Fungi; Saponin

Full Text:



G.J. Tortora, B.R. Funke, C.L. Case, Microbiology: An Introduction, 10th ed., 554-579, 758-759, Pearson Benjamin Cummings, San Fransisco. , 2010.

S. Hogg, Essential Microbiology, 365, John Wiley & Sons, Chichester, 2005.

S.P. Denyer, N.A. Hodges, S.P. Gorman, , Hugo and Russell’s Pharmaceutical Microbiology, 7th ed., 220-232, Blackwell Science, Victoria, 2004.

Anonim, Surveillance Report: Antimicrobial Resistance Surveillance in Europe 2009, European Centre for Disease Prevention and Control (ECDC), Stockholm, 2010.

S.T. Pratiwi, Mikrobiologi Farmasi, 154, 114-117, 188-192, Penerbit Erlangga, Jakarta, 2008.

T. Mohamed, S. Zhao, D.G. White, and S. Parveen, Molecular characterization of antibiotic resistant Salmonella typhimurium and Salmonella kentucky isolated from pre- and post-chill whole broilers carcasses. Food Microbiology, 38: pp. 6–15, 2014.

M. Iwamoto, Y. Mu, R. Lynfield, S.N. Bulens, J. Nadle, D. Aragon, D., Trends in invasive methicillin-resistant Staphylococcus aureus infections. Pediatrics, 132: pp. e817–824, 2013.

C.L. Ren, M.W. Konstan, A. Yegin, L. Rasouliyan, B.Trzaskoma, W.J. Morgan, Multiple antibiotic-resistant Pseudomonas aeruginosa and lung function decline in patients with cystic fibrosis. Journal of Cystic Fibrosis, 11. pp. 293–299, 2012.

M.A.N. Anikwe, K.C.A. Nwobodo, Long term effect of Municipal waste disposal on soil properties and productivity of sites used for urban agriculture in Abakaliki, Nigeria, Bioresource Technology, 83, pp. 241-250, 2002.

C. Crecchio, M. Curci, M.D.R. Pizzigallo, P. Ricciuti, P. Ruggierio, Effects of municipal solid waste compost amendments on soil enzyme activities and bacterial genetic diversity, Soil Biology & Chemistry, 36, pp. 1595-1605, 2004.

J.J. Lee, R.D. Park, Y.W. Kim, J.H. Shim, D.H. Chae, Y.S. Rim, B.K. Sohn, T.H. Kim, K.Y. Kim, Effect of food waste compost on microbial population, soil enzyme activity and lettuce growth, Bioresource Technology, 93, pp.21-28, 2004.

M. Lebkowska, E. Zborowska, E. Karwowska, E. Miaskiewicz-Peska, A. Muszynski, A. Tabernacka, J. Naumczyk, M. Jeczalik, Biremediation of soil polluted with fuels by sequential multiple injection of native microorganism: Field-scale processes in Poland, Ecological Engineering, 37, pp.1895-1900, 2011.

A.K. Karamalidis, A.C. Evangelou, E. Karabika, A.I. Koukkou, C. Drainas, E.A. Voudrias, Laboratory scale bioremediation of petroleum-contaminated soil by indigenous microorganisms and added Pseudomonas aeruginosa strain Spet, Bioresource Technology, 101, pp. 6545-6552, 2010.



  • There are currently no refbacks.

Copyright (c) 2017 Journal Of Natural Sciences And Mathematics Research