Antibacterial activity of actinomycetes associated with clove plant (Syzygium aromaticum) against Staphylococcus aureus and Escherichia coli
DOI:
https://doi.org/10.21580/jnsmr.2023.9.2.16702Keywords:
Antibacterial, actinomycetes, Escherichia coli, Syzygium aromaticum, Staphylococcus aureusAbstract
Actinomycetes form associations with plants through colonizing plant tissues (endophytes) or by residing in the soil around some plants' roots (rhizosphere). Actinomycetes are known to produce antibacterial compounds. This study aimed to investigate the antibacterial activity of actinomycetes associated with the clove plant (Syzygium aromaticum) against Staphylococcus aureus and Escherichia coli. Actinomycetes were isolated from clove plants and the rhizosphere, and their antimicrobial activity against Staphylococcus aureus and Escherichia coli was evaluated using the agar plug method, where the presence of transparent zones around 10-day-old actinomycete growth indicated inhibition of bacterial growth. Four isolates showed inhibitory effects against Staphylococcus aureus, while only three isolates, B.4, T.3, and T.4, demonstrated inhibitory activity against Escherichia coli, as indicated by the presence of inhibition zones. Isolate T.3 exhibited the highest inhibition zone of 8.5 mm against S. aureus, whereas B.4 displayed the highest inhibition zone of 7.7 mm against E. coli. In conclusion, the actinomycetes found in clove plants (Syzygium aromaticum) demonstrate antibacterial properties against Staphylococcus aureus and Escherichia coli, indicating their potential as antibacterial agents.
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Aamir, M., Rai, K., Zehra, A., Dubey, M., Samal, S., Yadav, M., & Upadhyay, R. (2020). Endophytic actinomycetes in bioactive compounds production and plant defense system. Microbial Endophytes, 189-229. https://doi.org/10.1016/B978-0-12-818734-0.00009-7
Aminingsih, T., Nashrianto, H., dan Rohman, A. J. (2012). Potensi Antibakteri Terhadap Escherichia coli dan Staphylococcus aureus dan Identifikasi Senyawa Ekstrak Heksana Bandotan (Ageratum conyzoides
L.). Fitofarmaka, 2(1): 18-26. https://doi.org/10.33751/jf.v2i1.163
Anandan, R., Dharumadurai, D and Manogaran, G. P. (2016). An Introduction to Actinobacteria, Actinobacteria - Basics and Biotechnological Applications, Dharumadurai Dhanasekaran and Yi Jiang, IntechOpen. https://doi.org/10.5772/62329
Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71–79. https://doi.org/10.1016/j.jpha.2015.11.005
Budhathoki, S., & Shrestha, A. (2020). Screening of Actinomycetes From Soil for Antibacterial Activity. Nepal Journal of Biotechnology, 8(3): 102-110. https://doi.org/10.3126/njb.v8i3.33664
Chen, J., Xu, L., Zhou, Y., & Han, B. (2021). Natural Products from Actinomycetes Associated with Marine Organisms. Marine Drugs, 19(11), 629. https://doi.org/10.3390/md19110629
Dadgostar, P. (2019). Antimicrobial Resistance: Implications and Costs. Infect Drug Resist, 12, 3903-3910. https://doi.org/10.2147/IDR.S234610
David W. W. and Stout T. R. (1971). Disc plate method of microbiological antibiotic assay. I. Factors influencing variability and error. Applied Microbiology, 22, 659-665.
De Simeis D, Serra S. Actinomycetes: A Never-Ending Source of Bioactive Compounds-An Overview on Antibiotics Production. Antibiotics (Basel), 10(5), 483. PMID: 33922100; PMCID: PMC8143475. https://doi.org/10.3390/antibiotics10050483
Elshafie, H. S., & Camele, I. (2022). Rhizospheric Actinomycetes Revealed Antifungal and Plant Growth-Promoting Activities under Controlled Environment. Plants (Basel, Switzerland), 11(14), 1872. https://doi.org/10.3390/plants11141872
Gajdács, M., & Albericio, F. (2019). Antibiotic Resistance: From the Bench to Patients. Antibiotics (Basel, Switzerland), 8(3), 129. https://doi.org/10.3390/antibiotics8030129
Guo, Y., Song, G., Sun, M., Wang, J., & Wang, Y. (2020). Prevalence and Therapies of Antibiotic Resistance in Staphylococcus aureus. Frontiers in Cellular and Infection Microbiology, 10, 107. https://doi.org/10.3389/fcimb.2020.00107
Jiang, Y., Li, Q., Chen, X., & Jiang, C. (2016). Isolation and cultivation methods of Actinobacteria. In D. Dhanasekaran & Y. Jiang (Eds.), Actinobacteria-Basics and Biotechnological Applications
(Vol. 2, pp. 39-57). InTech. https://doi.org/10.5772/61457
Katili A. & Retnowati Y. (2017). Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia. Biodiversitas Journal of Biological Diversity, 18(2), 826-833. https://doi.org/10.13057/biodiv/d180259
Klein, E. Y., Tseng, K. K., Pant, S., & Laxminarayan, R. (2019). Tracking global trends in the effectiveness of antibiotic therapy using the Drug Resistance Index. BMJ Global Health, 4(2), e001315. https://doi.org/10.1136/bmjgh-2018-001315
Lee, D. S., Lee, S. J., & Choe, H. S. (2018). Community-Acquired Urinary Tract Infection by Escherichia coli in the Era of Antibiotic Resistance. BioMed Research International, 2018, 7656752. https://doi.org/10.1155/2018/7656752
Li, Q., Chen, X., Jiang, Y and Jiang, C. 2016. Morphological Identification of Actinobacteria, Actinobacteria - Basics and Biotechnological Applications, Dharumadurai Dhanasekaran and Yi Jiang. IntechOpen. https://doi.org/10.5772/61461
Lubis, S. S. (2015). Penapisan Bakteri Laut Penghasil Antimikroba dari Pesisir Serdang Bedagai Sumatera Utara. Journal of Islamic Science and Technology,1(1): 87-96.
Mfoutou Mapanguy, C. C., Adedoja, A., Kecka, L. G. V., Vouvoungui, J. C., Nguimbi, E., Velavan, T. P., & Ntoumi, F. (2021). High prevalence of antibiotic-resistant Escherichia coli in Congolese students. International Journal of Infectious Diseases: IJID: Official Publication of the
International Society for Infectious Diseases, 103, 119–123.
https://doi.org/10.1016/j.ijid.2020.09.1441
Nalini, M. S., & Prakash, H. S. (2017). Diversity and bioprospecting of actinomycete endophytes from medicinal plants. Letters in Applied Microbiology, 64(4), 261–270. https://doi.org/10.1111/lam.12718
Natsagdorj, O., Bekh-Ochir, D., Baljinova, T., Iizaka, Y., Fukumoto, A., & Kato, F. et al. (2021). Bioactive compounds and molecular diversity of endophytic actinobacteria isolated from desert plants. IOP Conference Series: Earth And Environmental Science, 908(1), 012008.
https://doi.org/10.1088/1755-1315/908/1/012008
Oberhofer, M., Hess, J., Leutgeb, M., Gössnitzer, F., Rattei, T., Wawrosch, C., & Zotchev, S. B. (2019). Exploring Actinobacteria Associated With Rhizosphere and Endosphere of the Native Alpine Medicinal Plant Leontopodium nivale Subspecies alpinum. Frontiers in Microbiology, 10, 2531. https://doi.org/10.3389/fmicb.2019.02531
Ortlieb, N., Klenk, E., Kulik, A., & Niedermeyer, T. H. J. (2021). Development of an agar-plug cultivation system for bioactivity assays of actinomycete strain collections. PloS One, 16(11), e0258934. https://doi.org/10.1371/journal.pone.0258934
Passari, A. K., Mishra, V. K., Saikia, R., Gupta, V. K., & Singh, B. P. (2015). Isolation, abundance and phylogenetic affiliation of endophytic actinomycetes associated with medicinal plants and screening for their in vitro antimicrobial biosynthetic potential. Frontiers in Microbiology,
, 273. https://doi.org/10.3389/fmicb.2015.00273
Quach, N. T., Nguyen, Q. H., Vu, T. H. N., Le, T. T. H., Ta, T. T. T., Nguyen, T. D., Van Doan, T., Van Nguyen, T., Dang, T. T., Nguyen, X. C., Chu, H. H., & Phi, Q. T. (2021). Plant-derived bioactive compounds produced by Streptomyces variabilis LCP18 associated with Litsea cubeba (Lour.) Pers as potential target to combat human pathogenic bacteria and human cancer cell lines. Brazilian Journal of Microbiology: [Publication of the Brazilian Society for Microbiology], 52(3), 1215–1224. https://doi.org/10.1007/s42770-021-00510-6
Rozirwan, R., Muda, H., & Ulqodry, T. (2020). Short Communication: Antibacterial potential of Actinomycetes isolated from mangrove sediment in Tanjung Api-Api, South Sumatra, Indonesia. Biodiversitas
Journal of Biological Diversity, 21(12). https://doi.org/10.13057/biodiv/d211232
Saha, M., & Sarkar, A. (2021). Review on Multiple Facets of Drug Resistance: A Rising Challenge in the 21st Century. Journal of Xenobiotics, 11(4), 197–214. https://doi.org/10.3390/jox11040013
Saini, P., Gangwar, M., Kalia, A., Singh, N., & Narang, D. (2016). Isolation of endophytic actinomycetes from Syzygium cumini and their antimicrobial activity against human pathogens. Journal of Applied and Natural Science, 8(1), 416-422. https://doi.org/10.31018/jans.v8i1.809
Sarika, K., Sampath, G., Kaveriyappan Govindarajan, R., Ameen, F., Alwakeel, S., Al Gwaiz, H. I., Raja Komuraiah, T., & Ravi, G. (2021). Antimicrobial and antifungal activity of soil actinomycetes isolated from coal mine sites. Saudi Journal of Biological Sciences, 28(6), 3553–3558. https://doi.org/10.1016/j.sjbs.2021.03.029
Selim MSM, Abdelhamid SA, Mohamed SS. Secondary metabolites and biodiversity of actinomycetes. J Genet Eng Biotechnol, 19(1), 72. PMID: 33982192; PMCID: PMC8116480. https://doi.org/10.1186/s43141-021-00156-9
Seok, H., Choi, J. Y., Wi, Y. M., Park, D. W., Peck, K. R., & Ko, K. S. (2020). Fosfomycin Resistance in Escherichia coli Isolates from South Korea and in vitro Activity of Fosfomycin Alone and in Combination with Other Antibiotics. Antibiotics, 9(3), 112. MDPI AG. Retrieved from http://dx.doi.org/10.3390/antibiotics9030112
Sulis, G., Sayood, S., & Gandra, S. (2022). Antimicrobial resistance in low- and middle-income countries: current status and future directions. Expert Review of Anti-Infective Therapy, 20(2), 147–160. https://doi.org/10.1080/14787210.2021.1951705
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