Isolation and characterization of Bacteria in the ancient Javanese Pegon manuscript: “Layang Watu Gunung”

Authors

  • Tara Puri Ducha Rahmani Universitas Islam Negeri Walisongo Semarang, Indonesia
  • Nur Ahmad Leiden University, Netherlands
  • Anasom Anasom Universitas Islam Negeri Walisongo Semarang, Indonesia
  • Salsabiela Pertiwi Universitas Islam Negeri Walisongo Semarang, Indonesia
  • Muhammad Yusrun Niam Universitas Islam Negeri Walisongo Semarang, Indonesia
  • Hamdan Hadi Kusuma Universitas Islam Negeri Walisongo Semarang, Indonesia
  • Lee James Watson Chemtech Environmental Limited, Stanley, County Durham, United Kingdom

DOI:

https://doi.org/10.21580/jnsmr.v9i2.22679

Keywords:

biodeterioration, ancient manuscripts, preservation of manuscripts, microorganisms, pegon script

Abstract

This research is one of the efforts and the first step in the preservation of historical ancient manuscripts with an interdisciplinary scientific approach. The Unity of Sciences involved in this research are historical science, archaeology, philology combined with natural sciences such as microbiology and biotechnology approaches. The ancient manuscript whose microorganisms were isolated and observed in this study is an ancient manuscript with Pegon letters titled "Layang Watu Gunung" which dates from the 19th century. Over time, microorganisms can contaminate the paper on the manuscript which can cause damage or biodeterioration. Efforts to preserve historical manuscripts can be started by identifying in advance what types of microorganisms are contaminating the manuscript. From the results of the study, it was found that the microorganisms that contaminate the Layang Watu Gunung Ancient Manuscript are types of bacteria and fungi. For bacteria, the results of the Gram staining test result for contaminant bacteria are Gram Negative Bacteria. This initial research will be the basis for further preservation efforts, the results will be more relevant to be analyzed, especially for other historical manuscripts that are in similar temperature and humidity conditions, namely on the island of Java.

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Author Biographies

Tara Puri Ducha Rahmani, Universitas Islam Negeri Walisongo Semarang

Faculty of Science and Technology

Nur Ahmad, Leiden University

Leiden Institute for Area Studies

Anasom Anasom, Universitas Islam Negeri Walisongo Semarang

Faculty of Da'wa and Communication

Salsabiela Pertiwi, Universitas Islam Negeri Walisongo Semarang

Faculty of Science and Technology

Muhammad Yusrun Niam, Universitas Islam Negeri Walisongo Semarang

Faculty of Science and Technology

Hamdan Hadi Kusuma, Universitas Islam Negeri Walisongo Semarang

Faculty of Science and Technology

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Published

2023-12-29

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Section

Original Research Articles