Isolation of curcumin compounds in Temulawak Rhizome (Xanthorrhiza Roxb)

Muhammad Eka Putra Ramandha*  -  Universitas Bumigora, Indonesia
I Nyoman Bagus Aji Kresnapati  -  Universitas Bumigora, Indonesia

(*) Corresponding Author
The curcumin compounds in Temulawak Rhizome have been isolated and identified.. This study aims to identify curcumin compounds in temulawak rhizome by modifying methods that pay attention to the efficiency of funds and the use of materials. In general, the method of isolation and identification is carried out. The methods used are (1) Extraction, (2) Thin Layer Chromatography, (3) Column Chromatography and (4) Infrared (IR) Testing. Based on comparing the sample's Retention Factor (RF) value with the standard curcumin compound, the results were identical, and the positive sample contained a curcumin compound. The results of the Infrared spectrum can be assumed that the sample is a flavanols group, which can be seen from the wavelength range of identical functional groups in curcumin compounds.

Keywords: Curcumin; Temulawak Rhizome; Thin Layer; Chromatography; Infrared; Retention Factor

  1. Astuti A., Hendrarti W., & Wunas J. 2019. Synthesis and characterizations of absorbent dressing turmeric extract curcumin chitosan-alginate hydrogen and ZnO nano for mediate and high exudation. Ann Mechnikov’s Inst. 6(3): 1–9.
  2. Desai, N., Momin, M., Sigh, U., Khan, T., Sherje, A. 2019. Analytical Method Development and Validation For Simultaneous Estimation Of Curcumin And Cyclosporine By RP-HPLC. International Journal of Pharmacy and Pharmaceutical Sciences. 11(2): 26-33.
  3. Dicky A., Apriliana E. 2016. Efek Pemberian Ekstrak Temulawak (Curcuma Xanthorrhiza Roxb) terhadap Daya Hambat Pertumbuhan Staphylococcus Aureus dan Escherichia Coli Secara In Vitro. J Ked Univ Lampung.; 1(2). 308-312.
  4. Farida, Y., Rahmat, D., & Amanda, A.W. 2018. Uji Aktivitas Antiinflamasi Nanopartikel Ekstrak Etanol Rimpang Temulawak (Curcuma Xanthorrhiza Roxb.) dengan Metode Penghambatan Denaturasi Protein. Jurnal Ilmu Kefarmasian Indonesia, 16 (2),1693-1831
  5. Hakim, A., Idru, S, W, A., & Ramandha, M, E, P. 2020. Medicinal plant of sasambo as a traditional health tourism alternative post COVID-19. Journal Natural Resources. 11 (9).
  6. Haryani, F.,Hakim, A.& Hanifa, N, I. 2021. Perbandingan Pelarut Etanol 96% dan Aseton pada Ekstraksi dan Isolasi Kurkuminoid dari Rimpang Kunyit. Lumbung Farmasi; Jurnal Ilmu Kefarmasian. 2(2). 112-117.
  7. Hanwar, D., Handayani, V.R., Suhendi, A. 2020. Validasi Metode HPLC untuk Analisis Kurkumin pada Ekstrak Rimpang Temulawak (Curcuma xanthorrhiza Roxb.). Proceeding of The 12th University Research Colloquium 2020: Bidang MIPA dan Kesehatan. 12 Sep 2020. Surakarta,
  8. Jawa Tengah, Indonesia.
  9. Hirlekar, R., Khismatrao, A., Bhairy, S. 2018. Development And Validation Of RP-HPLC Method For Simultaneous Estimation Of Curcumin And Piperine. Innovare Academic Sciences Pvt. Ltd. India. 10,Issue-5. DOI: http://dx.doi.org/10.22159/ijap.2018v10i5.21140.
  10. Indriani, N., & Ramandha, M, E, P. 2023. Isolation Pinostrobin Compound In Temu Kunci (Kaempferia Pandurata Roxb) Rhizome. Jurnal Berkala Ilmiah Sains dan Terapan Kimia. 17(1). 29-34.
  11. Kautsari, S. N., Purwakusumah, E. D. & Nurcholis, W. 2020. Profil Kromatografi Lapis Tipis Ekstrak Kunyit (Curcuma longa Linn) Segar dan Simplisia Dengan Variasi Metode Ekstraksi. Media Farmasi, 16 (1), 65–70.
  12. Kesumayadi, I., Almas, AI., Rambe I,N,H, Hapsari R. 2021 Effect of Curcuma xanthorrhiza Gel on Methicillin-Resistant Staphylococcus aureus-Infected Second-Degree Burn Wound in Rats. Natural Product Sciences.; 27(1): 1–8.
  13. Lee, Y.S., Oh, S.M., Li, Q.Q., Kim, K.W., Yoon, D., Lee, M.H., Kwon, D.Y., Kang, O.H., Lee, D.Y. 2022. Validation of a Quantification Method for Curcumin Derivatives and Their Hepatoprotective Effects on Nonalcoholic Fatty Liver Disease. Molecular biology. 44(1): 409-432.
  14. Muhsin, L, B., & Ramandha, M, E, P. 2023. Ekstraksi Jahe (Zingiberis Officinale) dan uji pemisahan Kromatografi Lapis Tipis (KLT). Biocity Journal. 1(2). 66-72.
  15. Nadaf, S., Killedar, S. 2020. Development and Validation of RP–HPLC Method for Estimation of Curcumin from Nanocochleates and Its Application in in–vivo Pharmacokinetic Study. Acta Chimica Slovenica.67(4): 1100-1110.
  16. Nanda, E.V., Yopi, Y. Pratiwi, Y. 2021. Validasi dan Penetapan Kadar Kurkumin Pada Jamu Gendong Kunyit Asam dengan Metode Kromatografi Cair Kinerja Tinggi. Sainstech Farma: Jurnal Ilmu
  17. Kefarmasian. 14 (1): 12-18. DOI: https://doi.org/https://doi.org/10.37277/sfj.v14i1.932
  18. Panjaitan J, Girsang E, & Chiuman L. 2022. Effectiveness of temulawak (Curcuma xanthorrhiza) ointments as wound healing agents in wistar rats. J Teknol Lab.; 11(2): 95–104.
  19. Pawar, H. A., Gavasane, A. J. & Choudhary, P. D. 2018. A Novel and Simple Approach for Extraction and Isolation of Curcuminoids from Turmeric Rhizomes. Natural Products Chemistry & Research, 06 (01), 1–4.
  20. Ramandha, M, E, P., & Muhsin, L, B. 2023. Analisis uji pemisahan Parasetamol dan Vitamin C pada sediaan tablet Farmasi menggunakan uji pemisahan Kromatografi Lapis Tipis. Jurnal Ilmu Kesehatan dan Farmasi. 11(1). 16-19.
  21. Raut, R., Shaji, J. 2021. HPLC method validation for quantification of tetrahydrocurcumin in bulk drug and formulation. Futur J Pharm Sci. 7 (42). DOI: https://doi.org/10.1186/s43094-021-00194-7
  22. Risthanti, R. R., Sumiyani, R., Wulansari, D. D. & Anawati, T. J. 2019. Penetapan Kadar Kurkuminoid Dalam Ekstrak Campuran Curcuma domestica Val. dan Curcuma xanthorrhiza Roxb. Sebagai Bahan Baku Jamu Saintifik Secara KLT- Densitometri. Pharmaceutical. Journal of
  23. Indonesia, 5 (1), 37–43.
  24. Schiborr, C., Gunter, P. E., Rimbach, G., dan Frank, J. 2010. A validated method for the quantification of curcumin in plasma and brain tissue by fast narrow-bore high-performance liquid chromatography with fluorescence detection. University of Frankfurt. Germany. 397,1917
  25. DOI 10.1007/s00216-010- 3719-3
  26. Susanti, M., dan Dachriyanus. 2017. Kromatografi Cair Kinerja Tinggi. Lembaga Pengembangan Teknologi Informasi dan Komunikasi. Universtas Andalas. Padang.
  27. Susanto., Winarno, E.K. 2018. Penentuan Kadar Kurkumin Dari Beberapa Tanaman Curcuma Setelah IradiasiGamma. Prosiding Seminar Nasional APISORA 2018. 95-100.
  28. Syamsudin, R. A. M. R., Perdana, F., Mutiaz, F. S., Galuh, V., Rina, A. P. A., Cahyani, N. D.& Khendri, F. 2019. Temulawak Plant (Curcuma Xanthorrhiza Roxb.) As Traditional Medicine.Farmako Bahari, 10, 51-65.
  29. Tonnesen, H.H. and Karlsen, J.. 1985a. Studies on Curcuminand Curcuminoids, V. Alkaline Degradation of Curcumin, Z. Lebensm. Unters. Forsch. 180, 132-134.
  30. Tonnesen, H.H. and Karlsen, J.. 1985b. Studies on Curcuminand Curcuminoids, VI. Kinetics of Curcumin Degradation in Aqueous Solution, Z. Lebensm.Unters. Forsch.. 180, 402-404.
  31. Vikri, M., Sholih, M.G., Gatera, M.M. 2022. Identifikasi Kadar Kurkumin pada Minuman Serbuk Berbahan Temulawak dengan Metode Spektrofotometri Uv-Vis. Lumbung Farmasi; Jurnal
  32. Ilmu Kefarmasian. 3(2): 191-196.

Open Access Copyright (c) Journal of Natural Sciences and Mathematics Research
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Journal of Natural Sciences and Mathematics Research
Published by Faculty of Science and Technology
Universitas Islam Negeri Walisongo Semarang

Jl Prof. Dr. Hamka Kampus III Ngaliyan Semarang 50185
Website: https://journal.walisongo.ac.id/index.php/JNSMR
Email:jnsmr@walisongo.ac.id

ISSN: 2614-6487 (Print)
ISSN: 2460-4453 (Online)

View My Stats

Lisensi Creative Commons

This work is licensed under a Creative Commons Lisensi Creative Commons .

apps