Physical, Chemical, and Organoleptic Characteristics of Tempeh Cookies

Main Article Content

Nurul Hidayah
Amelya Aprilliani Yolanda

Abstract

Cookies are widely consumed snack options made primarily from wheat flour. Alternative materials, such as tempeh flour, are needed to replace wheat flour in the production of cookies. Tempeh contains high levels of protein and crude fiber. This research aims to determine the effect of wheat flour and tempeh flour formulations on the physical, chemical, and organoleptic characteristics of cookies. This research employed a one-factor Completely Randomized Design (CRD), with variations in wheat flour and tempeh flour (F1, 100% wheat flour: 0% tempeh flour), (F2, 75%: 25%), (F3, 50%: 50%), and (F4, 25%: 75%). A total of 31 untrained panelists carried out organoleptic evaluations on the product. The analyzed physical and chemical properties include moisture content, ash, protein, fat, carbohydrates, crude fiber, and texture. Organoleptic tests include taste, color, aroma, hardness, crispness, aftertaste, and overall impression. Data analysis was performed using One-way ANOVA at a 5% significance level, followed by Duncan's post-hoc test. Results indicate that the different formulations of wheat flour and tempeh flour significantly affect the moisture content, fat, protein, carbohydrates, crude fiber, hardness, crispness, and organoleptic properties of cookies. The best cookie formulation was obtained at F4 (25% wheat flour: 75% tempeh flour), with the highest protein and crude fiber content of 16.10% and 12.36%, respectively. This finding had implications for reducing dependence on imported wheat flour and encouraged the diversification of local food products based on fermented soybeans with high protein and fiber content.

Downloads

Download data is not yet available.

Article Details

How to Cite
Hidayah, N., & Yolanda, A. A. (2025). Physical, Chemical, and Organoleptic Characteristics of Tempeh Cookies. Nutri-Sains: Jurnal Gizi, Pangan Dan Aplikasinya, 9(1), 43–56. https://doi.org/10.21580/ns.2025.9.1.21538
Section
Articles
Author Biography

Nurul Hidayah, Universitas Ahmad Dahlan

Program Studi Teknologi Pangan

References

Bastian, F., Ishak, E., Tawali, A. B., & Bilang, M. (2013). Daya terima dan kandungan zat gizi formula Tepung Tempe dengan penambahan Semi Refined Carrageenan (SRC) dan Bubuk Kakao. Jurnal Aplikasi Teknologi Pangan, 2(1), 5–8. https://www.researchgate.net/publication/320944798_

BSN. (2011). Syarat mutu biskuit. Badan Standardisasi Nasional. http://www.bsn.go.id/

Diana, F. M. (2009). Fungsi dan metabolisme protein dalam tubuh manusia. Jurnal Kesehatan Masyarakat, 4(1), 47–52. https://jurnal.fkm.unand.ac.id/index.php/jkma/article/view/43

Gardjito, M., Djuwardi, A., & Harmayani, E. (2018). Pangan nusantara: karakteristik dan prospek untuk percepatan diversifikasi pangan. Prenada Media.

Hasibuan, S., Asben, A., & Andhika, D. (2024). Analysis of the comparison of Saga Seed Tempeh with Soybean Tempeh on the proximate quality value of the product. Asian Journal of Applied Research for Community for Community Development and Empowerment, 8(3), 128–131. https://doi.org/10.29165/ajarcde.v8i3.470

Istiqomah, I., Nurrahman, N., & Nurhidajah, N. (2019). Perubahan sifat kimia Tempe Kedelai Hitam dengan variasi kecambah dan lama inkubasi. Jurnal Penelitian Ilmu-Ilmu Teknologi Pangan, 8(1). https://jurnal.um-palembang.ac.id/edible/article/view/3448

Kaushik, R., Kumar, N., Sihag, M. K., & Ray, A. (2015). Isolation, characterization of wheat gluten and its regeneration properties. Journal of Food Science and Technology, 52(9), 5930–5937. https://doi.org/10.1007/s13197-014-1690-2

Kencanaputri, S. A., Ilmi, I. M. B., & Simanungkalit, S. F. (2024). Pengaruh keragaman pangan, junk food, dan produk tinggi gula terhadap kejadian gizi lebih remaja SMAN 6 Depok. Amerta Nutrition, 8(3SP), 115–126. https://doi.org/10.20473/amnt.v8i3sp.2024.115-126

Koni, T. N. I., Soi, R., & Foenay, T. A. Y. (2023). Content of dry matter, crude fiber, and ash in fermented rice bran with palmyra sap at different length of fermentation. BIO Web of Conferences, 81. https://doi.org/10.1051/bioconf/20238100035

Kristanti, D., Setiaboma, W., & Herminiati, A. (2020). Karakteristik fisikokimia Dan organoleptik Cookies Mocaf Dengan penambahan Tepung Tempe (Physicochemical and Organoleptic characteristics of Mocaf Cookies with Tempeh flour additions). Biopropal Industri, 11(1), 1–8. https://doi.org/10.36974/jbi.v11i1.5354

Kumalasari, I. D. (2024). Analysis of sensory acceptance of cookies made from Almond Flour (Prunus dulcis) and Sweet Corn Flour (Zea mays saccharata Sturt.). Agroindustrial Technology Journal, 8(1), 77–87. https://doi.org/10.21111/atj.v8i1.11123

Lailatul, H. N., & Anna, C. (2019). Pengaruh subsitusi Tepung Tempe dan penambahan margarin terhadap mutu organoleptik Kue Kembang Goyang. Jurnal Tata Boga, 8(I), 23–31. https://ejournal.unesa.ac.id/index.php/jurnal-tata-boga/article/view/26098

Madani, A., Fertiasari, R., Tritisari, A., & Safitri, N. (2023). Analisis kandungan Proksimat Cookies Tepung Tempe. Journal of Food Security and Agroindustry, 1(2), 40–49. https://doi.org/10.58184/jfsa.v1i2.87

Malau, M. S., Yusmarini, Y., & Johan, V. S. (2022). Pemanfaatan Tepung Pisang Kepok Dan Tepung Tempe dalam pembuatan kukis. Sagu, 21(2), 79. https://doi.org/10.31258/sagu.21.2.p.79-85

Marcelli, V., Osimani, A., & Aquilanti, L. (2024). Research progress in the use of lactic acid bacteria as natural biopreservatives against Pseudomonas spp. in meat and meat products: A review. Food Research International, 196(115129). https://doi.org/10.1016/j.foodres.2024.115129

Nariah, H., Kisnawaty, S. W., & Purwani, E. (2024). Kadar protein dan tingkat kekerasan pada Cookies Tepung Gaplek dan Tepung Tempe sebagai potensi terapi Celiac Disease. Ghidza: Jurnal Gizi Dan Kesehatan, 8(2), 232–241. https://doi.org/10.22487/ghidza.v8i2.1619

Nunes, E. A., Colenso-Semple, L., McKellar, S. R., Yau, T., Ali, M. U., Fitzpatrick-Lewis, D., Sherifali, D., Gaudichon, C., Tomé, D., Atherton, P. J., Robles, M. C., Naranjo-Modad, S., Braun, M., Landi, F., & Phillips, S. M. (2022). Systematic review and meta-analysis of protein intake to support muscle mass and function in healthy adults. Journal of Cachexia, Sarcopenia and Muscle, 13(2), 795–810. https://doi.org/10.1002/jcsm.12922

Rismayanthi, C. (2015). Sistem energi dan kebutuhan zat gizi yang diperlukan untuk peningkatan prestasi atlet. Jorpres (Jurnal Olahraga Prestasi), 11(1). https://doi.org/10.21831/jorpres.v11i1.10270

Safitri, N. R. D., & Fitranti, D. Y. (2016). Pengaruh edukasi gizi dengan ceramah dan booklet terhadap peningkatan pengetahuan dan sikap gizi remaja overweight. Journal of Nutrition College, 5(4), 374–380. https://doi.org/10.14710/jnc.v5i4.16438

Salim, R., Zebua, E. T., & Taslim, T. (2017). Analisis jenis kemasan terhadap kadar protein dan kadar air pada tempe. Jurnal Katalisator, 2(2), 106–111. https://doi.org/10.22216/jk.v2i2.2531

Sari, L. N., Rowa, S. S., & Suaib, F. (2019). Biscuit with substitution Of Red Bean Flour and Taro Flour. Media Gizi Pangan, 26(1), 37. https://doi.org/10.32382/mgp.v26i1.473

Seveline, S., Diana, N., & Taufik, M. (2019). Formulasi cookies dengan fortifikasi Tepung Tempe dengan penambahan Rosela (Hibiscus sabdariffa L.) formulation of cookies fortified with Tempeh flour and addition of Rosele (Hibiscus sabdariffa L.). Jurnal Bioindustri, 1(2), 245–260. https://doi.org/10.31326/jbio.v1i2.78

Silalahi, J. (2006). Makanan fungsional. Kanisius. https://books.google.co.id/books/about/Makanan_Fungsional.html?id=bQjyzQEACAAJ&redir_esc=y

Sipayung, E. N., Herawati, N., & Rahmayuni. (2014). Potensi Tepung Ubi Jalar Ungu (Ipomoea babatas I.), Tepung Tempe, dan Tepung Udang Rebon dalam Pembuatan Cookies. Jurnal Online Mahasiswa, 1(1). https://jom.unri.ac.id/index.php/JOMFAPERTA/article/view/2628

Soerjodibroto. (2004). Asupan serat makan remaja di Jakarta. Majalah Kedokteran Indonesia.

Teoh, S. Q., Chin, N. L., Chong, C. W., Ripen, A. M., How, S., & Lim, J. J. L. (2024). A review on health benefits and processing of tempeh with outlines on its functional microbes. Future Food, 9, 100330. https://doi.org/10.1016/j.fufo.2024.100330

Turana, Y., Handajani, Y. S., Barus, T., Kristian, K., Theodoraliu, E., & Suswanti, I. (2025). Comparison of the effects of mixed tempeh with soy tempeh on cognitive function in older people. Frontiers in Nutrition, 12(1551211). https://doi.org/10.3389/fnut.2025.1551211