Pengaruh Sinbiotik Kefir Tepung Pisang Batu (Musa balbisiana) terhadap Kadar Kolesterol Total dan Kadar Trigliserida Tikus Model Sindrom Metabolik

Diana Martha Ariesta Sari*  -  Universitas Pembangunan Nasional "Veteran" Jakarta, Indonesia
Taufik Maryusman  -  Universitas Pembangunan Nasional "Veteran" Jakarta, Indonesia
Santi Herlina  -  Universitas Pembangunan Nasional "Veteran" Jakarta, Indonesia

(*) Corresponding Author
DOI: 10.21580/ns.2020.4.1.4335
This study was conducted to analyze the effects of banana (Musa balbisiana) flour kefir synbiotic on total cholesterol level and triglyceride level of Metabolic Syndrome rats model. This research used a true experimental method with pre-post test control group design. The number of 24 rats model were randomly divided into four groups, i.e. K– (healthy rats, standard diet), K+ (Metabolic Syndrome rats, standard diet), PI (Metabolic Syndrome rats, standard diet and synbiotic 1,8 ml/200grBW/day), and PII (Metabolic Syndrome rats, standard diet and synbiotic 3,6 ml/200grBW/day), with intervention duration of 3 weeks. Blood draws were carried out at the beginning and end session of the intervention to analyze total cholesterol and triglyceride levels. The data from the measurement of total cholesterol and triglyceride levels before and after the intervention were then analyzed using the Paired T-Test. The results of the Paired T-Test showed differences (p<0,05) in total cholesterol and triglyceride levels before and after intervention in all groups. The banana flour kefir synbiotic has been shown to reduce cholesterol and triglyceride levels in rats.

Penelitian ini dilakukan untuk menganalisis pengaruh sinbiotik kefir tepung pisang batu terhadap kadar kolesterol total dan kadar trigliserida tikus model sindrom metabolik. Desain penelitian ini eksperimental murni dengan pre-post test control group design. Sebanyak 24 tikus Sprague dawley jantan dibagi secara acak menjadi empat kelompok, yaitu K– (tikus sehat, pakan standar), K+ (tikus sindrom metabolik, pakan standar), PI (tikus sindrom metabolik, pakan standar dan sinbiotik 1,8 ml/200gr BB tikus/hari), dan PII (tikus sindrom metabolik, pakan standar dan sinbiotik 3,6 ml/200gr BB tikus/hari) dengan intervensi 3 minggu. Pengambilan darah dilakukan pada awal dan akhir intervensi untuk menganalisis kadar kolesterol total dan trigliserida. Kadar kolesterol total dan trigliserida sebelum dan setelah intervensi dianalisis dengan uji Paired T-Test. Hasil penelitian menunjukkan bahwa terdapat perbedaan nyata (p<0,05) kadar kolesterol total dan kadar trigliserida sebelum dan setelah intervensi pada semua kelompok. Sinbiotik kefir tepung pisang batu terbukti mampu menurunkan kadar kolesterol dan trigliserida tikus.

Keywords: Synbiotic; kefir; Musa balbisiana; cholesterol; triglyceride; Sinbiotik; kefir; pisang batu (Musa balbisiana); kolesterol; trigliserida

  1. Ahmed, A. S., El-Bassiony, T., Elmalt, L. M., & Ibrahim, H. R. (2015). Identification of potent antioxidant bioactive peptides from goat milk proteins. Food Research International, 74, 80–88. https://doi.org/10.1016/j.foodres.2015.04.032
  2. Andini, A. N., & Ardiaria, M. (2016). Pengaruh pemberian kombinasi minyak rami dengan minyak wijen terhadap kadar trigliserida pada tikus sprague dawley dislipidemia. Journal of Nutrition College, 5(4), 555–564. https://doi.org/10.14710/jnc.v5i4.16472
  3. Blesso, C. N., & Fernandez, M. L. (2018). Dietary cholesterol, serum lipids, and heart disease: are eggs working for or against you? Nutrients, 10(426), 1–12. https://doi.org/10.3390/nu10040426
  4. Bourrie, B. C. T., Willing, B. P., & Cotter, P. D. (2016). The Microbiota and Health Promoting Characteristics of the Fermented Beverage Kefir. Frontiers in Microbiology, 7(647), 1–17. https://doi.org/10.3389/fmicb.2016.00647
  5. Cahyani, T. D. A. (2017). Pengaruh yoghurt dan soyghurt herbal sinbiotik jelly drink ekstrak jahe merah (zingiber officinale var. rubrum) terhadap kadar malondialdehid (mda) tikus pra-sindrom metabolik. Universitas Diponegoro, Semarang.
  6. Carasi, P., Racedo, S. M., Jacquot, C., Romanin, D. E., Serradell, M. A., & Urdaci, M. C. (2015). Impact of kefir derived lactobacillus kefiri on the mucosal immune response and gut microbiota. Journal of Immunology Research, 2015, 1–12. https://doi.org/10.1155/2015/361604
  7. Chen, C.-Y., Lee, C.-W., Chien, S.-C., Su, M.-I., Lin, S.-I., Cheng, C.-W., … Yeh, H.-I. (2018). Dyslipidemia management for elderly people with metabolic syndrome: A mini-review. International Journal of Gerontology, 12(1), 7–11. https://doi.org/10.1016/j.ijge.2017.07.001
  8. Cho, Y. A., & Kim, J. (2015). Effect of probiotics on blood lipid concentrations: A meta-analysis of randomized controlled trials. Medicine, 94(43), 1–10. https://doi.org/10.1097/MD.0000000000001714
  9. Crescenzo, R., Bianco, F., Coppola, P., Mazzoli, A., Tussellino, M., Carotenuto, R., … Iossa, S. (2014). Fructose supplementation worsens the deleterious effects of short-term high-fat feeding on hepatic steatosis and lipid metabolism in adult rats. Experimental Physiology, 99(9), 1203–1213. https://doi.org/10.1113/expphysiol.2014.079632
  10. Febriansyah, R., & Pramono, A. (2015). Pengaruh pemberian yoghurt sinbiotik tanpa lemak dengan penambahan tepung gembili terhadap kadar trigliserida tikus hiperkolesterolemia. Journal of Nutrition College, 4(1), 57–61. https://doi.org/10.14710/jnc.v4i1.8621
  11. Ferrarese, R., Ceresola, E. R., Preti, A., & Canducc, F. (2018). Probiotics, prebiotics and synbiotics for weight loss and metabolic syndromein the microbiome era. European Review for Medical and Pharmacological Sciences, 22, 7588–7605. https://doi.org/10.26355/eurrev_201811_16301
  12. García, V., Rovira, S., Boutoial, K., & López, M. B. (2014). Improvements in goat milk quality: A review. Small Ruminant Research, 121(1), 51–57. https://doi.org/10.1016/j.smallrumres.2013.12.034
  13. Gropper, S. S., & Smith, ‎Jack L. (2013). Nutrition and human metabolism (6th ed.). USA: Wadsworth.
  14. Hardiansyah, A. (2020). IDENTIFIKASI NILAI GIZI DAN POTENSI MANFAAT KEFIR SUSU KAMBING KALIGESING. Journal of Nutrition College, 9(3), 208–214.
  15. Hardisari, R., & Amaliawati, N. (2016). Manfaat Prebiotik Tepung Pisang Kepok (Musa paradisiaca formatypica) terhadap Pertumbuhan Probiotik Lactobacillus casei secara In Vitro. Jurnal Teknologi Laboratorium, 5(2), 64–67.
  16. Hidayati, S. N., & Syauqy, A. (2015). Pengaruh pemberian pisang kepok (musa paradisiacal forma typical) terhadap kadar kolesterol total tikus sprague dawley pra sindrom metabolik. Journal of Nutrition College, 4(4), 499–507. https://doi.org/10.14710/jnc.v4i4.10154
  17. Markowiak, P., & Śliżewska, K. (2017). Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients, 1021(9), 1–30. https://doi.org/10.3390/nu9091021
  18. Mazidi, M., Pennathur, S., & Afshinnia, F. (2017). Link of dietary patterns with metabolic syndrome: Analysis of the national health and nutrition examination survey. Nutrition & Diabetes, 7(3), 1–8. https://doi.org/10.1038/nutd.2017.11
  19. McLoughlin, R. F., Berthon, B. S., Jensen, M. E., Baines, K. J., & Wood, L. G. (2017). Short-chain fatty acids, prebiotics, synbiotics, and systemic inflammation: A systematic review and meta-analysis. The American Journal of Clinical Nutrition, 106(3), 930–945. https://doi.org/10.3945/ajcn.117.156265
  20. Moroti, C., Souza Magri, L., de Rezende Costa, M., Cavallini, D. C., & Sivieri, K. (2012). Effect of the consumption of a new symbiotic shake on glycemia and cholesterol levels in elderly people with type 2 diabetes mellitus. Lipids in Health and Disease, 11(29), 1–8. https://doi.org/10.1186/1476-511X-11-29
  21. Mukaromah, A. S., & Susilowati, F. (2021). Tropical Herbs and Spices as Functional Foods with Antidiabetic activity. USA: Apple Academic Press.
  22. Musita, N. (2014). Pemanfaatan tepung pisang batu (musa balbisiana colla) pada pembuatan kue brownies. Indonesian Journal of Industrial Research, 8(3), 171–178.
  23. Nolan, P. B., Carrick-Ranson, G., Stinear, J. W., Reading, S. A., & Dalleck, L. C. (2017). Prevalence of metabolic syndrome and metabolic syndrome components in young adults: A pooled analysis. Preventive Medicine Reports, 7, 211–215. https://doi.org/10.1016/j.pmedr.2017.07.004
  24. Nova, M., & Yanti, R. (2017). Faktor-faktor yang berhubungan dengan obesitas pada orang dewasa di Kota Padang Panjang. Nutri-Sains: Jurnal Gizi, Pangan Dan Aplikasinya, 1(1), 12–21.
  25. Nurliyani, Harmayani, E., & Sunarti. (2018). Goat milk kefir supplemented with porang glucomannan improves lipid profile and haematological parameter in rat fed high fat and high fructose diet. Romanian Journal of Diabetes Nutrition and Metabolic Diseases, 25(1), 11–21. https://doi.org/10.2478/rjdnmd-2018-0002
  26. Octavia, Z. F., Djamiatun, K., & Suci, N. (2017). Pengaruh pemberian yogurt sinbiotik tepung pisang tanduk terhadap profil lipid tikus sindrom metabolik. Jurnal Gizi Klinik Indonesia, 13(4), 159–169. https://doi.org/10.22146/ijcn.19369
  27. Pandey, K. R., Naik, S. R., & Vakil, B. V. (2015). Probiotics, prebiotics and synbiotics- a review. Journal of Food Science and Technology, 52(12), 7577–7587. https://doi.org/10.1007/s13197-015-1921-1
  28. Pimenta, F. S., Luaces-Regueira, M., Ton, A. M., Campagnaro, B. P., Campos-Toimil, M., Pereira, T. M., & Vasquez, E. C. (2018). Mechanisms of action of kefir in chronic cardiovascular and metabolic diseases. Cellular Physiology and Biochemistry, 48(5), 1901–1914. https://doi.org/10.1159/000492511
  29. Praba, S. K. (2019). Formulasi kefir susu kambing dengan penambahan tepung pisang batu (musa balbisiana) sebagai minuman sinbiotik. Universitas Pembangunan Nasional Veteran Jakarta, Jakarta.
  30. Rahmawati, F. C., Djamiatun, K., & Suci, N. (2017). Pengaruh yogurt sinbiotik pisang terhadap kadar glukosa dan insulin tikus sindrom metabolik. Jurnal Gizi Klinik Indonesia, 14(1), 11–21. https://doi.org/10.22146/ijcn.19379
  31. Razmpoosh, E., Javadi, A., Ejtahed, H. S., Mirmiran, P., Javadi, M., & Yousefinejad, A. (2019). The effect of probiotic supplementation on glycemic control and lipid profile in patients with type 2 diabetes: A randomized placebo controlled trial. Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 13(1), 175–182. https://doi.org/10.1016/j.dsx.2018.08.008
  32. Rosa, D. D., Dias, M. M. S., Grześkowiak, Ł. M., Reis, S. A., Conceição, L. L., & Peluzio, M. do C. G. (2017). Milk kefir : Nutritional, microbiological and health benefits. Nutrition Research Reviews, 30(1), 82–96. https://doi.org/10.1017/S0954422416000275
  33. Rusdaina, R., & Syauqy, A. (2015). Pengaruh pemberian pisang kepok (musa paradisiaca forma typical) terhadap kadar trigliserida tikus sprague dawley pra sindrom metabolik. Journal of Nutrition College, 4(4), 585–592. https://doi.org/10.14710/jnc.v4i4.10166
  34. Saklayen, M. G. (2018). The global epidemic of the metabolic syndrome. Current Hypertension Reports, 20(2), 1–8. https://doi.org/10.1007/s11906-018-0812-z
  35. Saputra, S., & Margawati, A. (2015). Pengaruh pemberian yoghurt sinbiotik tanpa lemak dengan penambahan tepung gembili (dioscorea esculenta) terhadap kadar kolesterol total tikus hiperkolesterolemia. Journal of Nutrition College, 4(2), 104–109. https://doi.org/10.14710/jnc.v4i2.10052
  36. Singh, A., & Singh, R. (2016). Triglyceride and cardiovascular risk: A critical appraisal. Indian Journal of Endocrinology and Metabolism, 20(4), 418-428. https://doi.org/10.4103/2230-8210.183460
  37. Soewondo, P., Purnamasari, D., Oemardi, M., Waspadji, S., & Soegondo, S. (2010). Prevalence of metabolic syndrome using NCEP/ATP III criteria in Jakarta, Indonesia: the Jakarta primary non-communicable disease risk factors surveillance 2006. Acta Medica Indonesiana, 42(4), 199–203.
  38. Syagata, A. (2019). Risiko Tinggi Diabetes Mellitus Tipe 2 pada Pegawai Usia Dewasa (High Risk of Type 2 Diabetes Mellitus among Working Adult). 3, 52–59.
  39. Weitkunat, K., Schumann, S., Petzke, K. J., Blaut, M., Loh, G., & Klaus, S. (2015). Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice. The Journal of Nutritional Biochemistry, 26(9), 929–937. https://doi.org/10.1016/j.jnutbio.2015.03.010
  40. World Health Organization (WHO). (2000). General guidelines for methodologies on research and evaluation of traditional medicine. Geneva: World Health Organization (WHO).
  41. Wu, Y., Zhang, Q., Ren, Y., & Ruan, Z. (2017). Effect of probiotic Lactobacillus on lipid profile: A systematic review and meta-analysis of randomized, controlled trials. PLOS ONE, 12(6), 1–15. https://doi.org/10.1371/journal.pone.0178868
  42. Zhang, A., Yao, Y., Xue, Z., Guo, X., Dou, J., Lv, Y., … Jin, L. (2018). A study on the factors influencing triglyceride levels among adults in Northeast China. Scientific Reports, 8(1), 1–7. https://doi.org/10.1038/s41598-018-24230-4
  43. Zhuhua, Z., Zhiquan, W., Zhen, Y., Yixin, N., Weiwei, Z., Xiaoyong, L., … Qing, S. (2015). A novel mice model of metabolic syndrome: the high-fat-high-fructose diet-fed ICR mice. Experimental Animals, 64(4), 435–442. https://doi.org/10.1538/expanim.14-0086

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